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Literature summary extracted from

  • Amir, J.; Cherry, J.H.
    Purification and properties of adenosine diphosphoglucose pyrophosphorylase from sweet corn (1972), Plant Physiol., 49, 893-897.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate allosteric activation Zea mays
2.7.7.27 3-phosphoglycerate activation Zea mays
2.7.7.27 D-fructose 6-phosphate activation Zea mays
2.7.7.27 D-fructose 6-phosphate less effective than 3-phosphoglycerate Zea mays
2.7.7.27 D-ribose 5-phosphate activation Zea mays
2.7.7.27 D-ribose 5-phosphate less effective than 3-phosphoglycerate Zea mays

General Stability

EC Number General Stability Organism
2.7.7.27 freeze-thawing reduces activity by 70%, high ionic strength, ATP and Mg2+ stabilize Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP
-
 Zea mays
2.7.7.27 AMP
-
 Zea mays
2.7.7.27 Cl- not at 5 mM, only at 10 mM Zea mays
2.7.7.27 diphosphate
-
 Zea mays
2.7.7.27 F- not at 5 mM, only at 10 mM Zea mays
2.7.7.27 NO3- not at 5 mM, only at 10 mM Zea mays
2.7.7.27 phosphate allosteric inhibitor Zea mays
2.7.7.27 phosphoenolpyruvate
-
 Zea mays
2.7.7.27 pyruvate
-
 Zea mays
2.7.7.27 SO42- 5 mM, weak Zea mays
2.7.7.27 UDP-glucose
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.032
-
 ATP pH 7.4, 37°C Zea mays
2.7.7.27 0.033
-
 diphosphate pH 7.4, 37°C Zea mays
2.7.7.27 0.19
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C Zea mays
2.7.7.27 0.62
-
 ADP-glucose pH 7.4, 37°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Zea mays
2.7.7.27 Mg2+ 6-8 mM Zea mays
2.7.7.27 additional information no activation by Mn2+, Zn2+, NH4+, Na+ Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Zea mays key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Zea mays
-
 sweet corn, var. Golden Beauty
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 seed
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 4.4
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 -20°C, in 60% (NH4)2SO4, 15 weeks Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate specific for ATP Zea mays diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate not: TTP Zea mays diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate no substrate is UTP Zea mays diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Zea mays diphosphate + ADP-glucose
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 7 7.5 ADP-glucose synthesis, Tris buffer Zea mays
2.7.7.27 7 8 ADP-glucose synthesis, HEPES buffer Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.27 6 8.3 about half-maximal activity at pH 6.0 and 8.3, Tris buffer Zea mays
2.7.7.27 6.1 8.5 about half-maximal activity at pH 6.1 and about 80% of maximal activity at pH 8.5, HEPES buffer Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 0.00038
-
 diphosphate pH 7.4, 37°C Zea mays