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Literature summary extracted from

  • Wang-Gillam, A.; Pastuszak, I.; Elbein, A.D.
    A 17-amino acid insert changes UDP-N-acetylhexosamine pyrophosphorylase specificity from UDP-GalNAc to UDP-GlcNAc (1998), J. Biol. Chem., 273, 27055-27057.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 AGX1 and AGX 2 expressed in Escherichia coli as GST fusion protein Homo sapiens
2.7.7.83 GST fusion protein expressed in Escherichia coli JM109 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.23 0.38
-
 N-acetyl-alpha-D-galactosamine 1-phosphate AGX1, 37°C, pH 7.5 Homo sapiens
2.7.7.23 1.3
-
 N-acetyl-alpha-D-galactosamine 1-phosphate AGX2, 37°C, pH 7.5 Homo sapiens
2.7.7.83 0.24
-
 N-acetyl-D-glucosamine 1-phosphate GST-fusion protein, N-acetylglucosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 0.32
-
 N-acetyl-D-glucosamine 1-phosphate GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 0.38
-
 N-acetyl-D-galactosamine 1-phosphate GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 1.3
-
 N-acetyl-D-galactosamine 1-phosphate GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.23 cytoplasm
-
 Homo sapiens 5737
-
2.7.7.23 cytoplasm
-
 Sus scrofa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.23 57000
-
 SDS-PAGE, two bands Sus scrofa
2.7.7.23 57000
-
 SDS-PAGE, AGX1 expressed in Escherichia coli Homo sapiens
2.7.7.23 64000
-
 SDS-PAGE, two bands Sus scrofa
2.7.7.23 64000
-
 SDS-PAGE, AGX2 expressed in Escherichia coli Homo sapiens
2.7.7.83 57000
-
 x * 57000, SDS-PAGE Homo sapiens
2.7.7.83 64000
-
 x * 64000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Sus scrofa amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Homo sapiens
-
-
-
2.7.7.23 Sus scrofa
-
-
-
2.7.7.83 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 GST fusion protein from E. coli Homo sapiens
2.7.7.83 affinity chromatography (glutathione-Sepharose), GST part removed Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.23 liver
-
 Sus scrofa
-
2.7.7.23 sperm
-
 Homo sapiens
-
2.7.7.23 testis
-
 Homo sapiens
-
2.7.7.83 testis
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.83 0.119
-
 GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, GST protein shows 0.02 micromol/min/mg in the assay, pH 7.5, 37°C Homo sapiens
2.7.7.83 0.3
-
 GST fusion removed, N-acetyl-D-galactosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 0.525
-
 GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, GST protein shows 0.033 micromol/min/mg in the assay, pH 7.5, 37°C Homo sapiens
2.7.7.83 0.887
-
 GST-fusion protein, N-acetyl-D-glucosamine 1-phosphate, GST protein shows 0.033 micromol/min/mg in the assay, pH 7.5, 37°C Homo sapiens
2.7.7.83 1.342
-
 GST-fusion protein, N-acetyl-D-galactosamine 1-phosphate, GST protein shows 0.02 micromol/min/mg in the assay, pH 7.5, 37°C Homo sapiens
2.7.7.83 5.873
-
 GST fusion removed, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 5.919
-
 GST fusion removed, N-acetyl-D-glucosamine 1-phosphate, pH 7.5, 37°C Homo sapiens
2.7.7.83 14.9
-
 GST fusion removed, N-acety-D-lgalactosamine 1-phosphate, pH 7.5, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-galactosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-galactosamine 2-3 times more active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate, AGX2 8 times less active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
 Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetyl-D-galactosamine
-
 ?
2.7.7.83 UTP + N-acetyl-D-galactosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetyl-D-galactosamine
-
 r
2.7.7.83 UTP + N-acetyl-D-glucosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetyl-D-glucosamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.23 dimer 1 * 64000 + 1 * 57000 Sus scrofa
2.7.7.83 ? x * 57000, SDS-PAGE Homo sapiens
2.7.7.83 ? x * 64000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.23 AGX1 from human sperm, 2-3 times more active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens
2.7.7.23 AGX2 identical sequence to AGX1 except a 17 amino acid insert at C-terminus, 8 times less active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens
2.7.7.83 UDP-N-acetylhexosamine pyrophosphorylase AGX1 isoform of AGX2 Homo sapiens
2.7.7.83 UDP-N-acetylhexosamine pyrophosphorylase AGX2 isoform of AGX1 Homo sapiens