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Literature summary extracted from

  • Wahle, E.
    Purification and characterization of a mammalian polyadenylate polymerase involved in the 3 end processing of messenger RNA precursors (1991), J. Biol. Chem., 266, 3131-3139.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.0036
-
 poly(A)n Mn2+-activated enzyme, pH 8.3, 37°C Bos taurus
2.7.7.19 0.01
-
 oligo(A)n Mn2+-activated enzyme, pH 8.3, 37°C Bos taurus
2.7.7.19 0.14 0.36 poly(A)n Mg2+-activated enzyme, pH 8.3, 37°C Bos taurus
2.7.7.19 0.3
-
 oligo(A)n Mg2+-activated enzyme, pH 8.3, 37°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 KCl requirement is dependent on the primer and the divalent cation used Bos taurus
2.7.7.19 Mg2+ more active in presence of Mn2+ than Mg2+ Bos taurus
2.7.7.19 Mg2+ optimal concentration: 4-6 mM Bos taurus
2.7.7.19 Mn2+ more active in presence of Mn2+ than Mg2+ Bos taurus
2.7.7.19 Mn2+ optimal concentration: 0.5 mM (at 0.5 mM ATP) Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 57000
-
 and 60000, 2 major forms of enzyme, gel filtration Bos taurus
2.7.7.19 60000
-
 and 57000, 2 major forms of enzyme, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.19 ATP + RNA Bos taurus involved in the 3'-end processing of mRNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Bos taurus
-
 calf
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.19 additional information not glycosylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 calf Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.7.19 -80°C, storage for 5 days including 2 cycles of freezing and thawing results in 35% inactivation Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + oligo(A)n
-
 Bos taurus diphosphate + oligo(A)n+1
-
 ?
2.7.7.19 ATP + poly(A)n
-
 Bos taurus diphosphate + poly(A)n+1
-
 ?
2.7.7.19 ATP + RNA elongation of the primer is distributive Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA involved in the 3'-end processing of mRNA Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8
-
 Mg2+-activated enzyme Bos taurus
2.7.7.19 8.3
-
 Mn2+-activated enzyme Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.19 7.2 9.2 pH 7.2: 55% of maximum activity, pH 9.2: 62% of maximum activity Bos taurus