EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.19 | diphosphate | - |
Rattus norvegicus | |
2.7.7.19 | additional information | not inhibitory: alpha-amanitin | Rattus norvegicus | |
2.7.7.19 | NH4+ | ammonium sulfate; maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus | |
2.7.7.19 | rifamycin AF/013 | O-n-octyloxime of 3-formylrifamycin SV | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.19 | 0.028 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.19 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.19 | KCl | maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus | |
2.7.7.19 | Mg2+ | more active in presence of Mn2+ than Mg2+ | Rattus norvegicus | |
2.7.7.19 | Mn2+ | required | Rattus norvegicus | |
2.7.7.19 | Mn2+ | more active in presence of Mn2+ than Mg2+ | Rattus norvegicus | |
2.7.7.19 | Mn2+ | optimal concentration: 0.25-1.0 mM | Rattus norvegicus | |
2.7.7.19 | NH4+ | maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.19 | 60000 | - |
mitochondria, glycerol density gradient centrifugation | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.19 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.19 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.19 | hepatoma cell | Morris hepatoma tumor cells 3924A | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.19 | additional information | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.19 | -90°C, 50% glycerol, stable for 4 weeks, about 40% loss of activity after 5 months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.19 | ATP + RNA | highly specific for ATP | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
2.7.7.19 | ATP + RNA | primer required | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
2.7.7.19 | ATP + RNA | poly(A) is the most effective primer | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
2.7.7.19 | ATP + RNA | mitochondrial RNA at least five times more efficiently used than nuclear RNA | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.19 | monomer | 1 * 60000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.19 | 7.8 | 8.2 | - |
Rattus norvegicus |