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Literature summary extracted from

  • Rose, K.M.; Morris, H.P.; Jacob, S.T.
    Mitochondrial poly(A) polymerase from a poorly differentiated hepatoma: purification and characteristics (1975), Biochemistry, 14, 1025-1032.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 diphosphate
-
 Rattus norvegicus
2.7.7.19 additional information not inhibitory: alpha-amanitin Rattus norvegicus
2.7.7.19 NH4+ ammonium sulfate; maximal activity at 33 mM, inhibition above 150 mM Rattus norvegicus
2.7.7.19 rifamycin AF/013 O-n-octyloxime of 3-formylrifamycin SV Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.028
-
 ATP 37°C, pH 8.0 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 KCl maximal activity at 33 mM, inhibition above 150 mM Rattus norvegicus
2.7.7.19 Mg2+ more active in presence of Mn2+ than Mg2+ Rattus norvegicus
2.7.7.19 Mn2+ required Rattus norvegicus
2.7.7.19 Mn2+ more active in presence of Mn2+ than Mg2+ Rattus norvegicus
2.7.7.19 Mn2+ optimal concentration: 0.25-1.0 mM Rattus norvegicus
2.7.7.19 NH4+ maximal activity at 33 mM, inhibition above 150 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 60000
-
 mitochondria, glycerol density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 hepatoma cell Morris hepatoma tumor cells 3924A Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.7.19 -90°C, 50% glycerol, stable for 4 weeks, about 40% loss of activity after 5 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Rattus norvegicus diphosphate + RNA(A)n average length of poly(A) formed is 600 nucleotides ?
2.7.7.19 ATP + RNA primer required Rattus norvegicus diphosphate + RNA(A)n average length of poly(A) formed is 600 nucleotides ?
2.7.7.19 ATP + RNA poly(A) is the most effective primer Rattus norvegicus diphosphate + RNA(A)n average length of poly(A) formed is 600 nucleotides ?
2.7.7.19 ATP + RNA mitochondrial RNA at least five times more efficiently used than nuclear RNA Rattus norvegicus diphosphate + RNA(A)n average length of poly(A) formed is 600 nucleotides ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 monomer 1 * 60000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 7.8 8.2
-
 Rattus norvegicus