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Literature summary extracted from

  • Olland, A.M.; Underwood, K.W.; Czerwinski, R.M.; Lo, M.C.; Aulabaugh, A.; Bard, J.; Stahl, M.L.; Somers, W.S.; Sullivan, F.X.; Chopra, R.
    Identification, characterization, and crystal structure of Bacillus subtilis nicotinic acid mononucleotide adenylyltransferase (2002), J. Biol. Chem., 277, 3698-3707.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.18 overexpression in Escherichia coli BL21 with a His-tag Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.18 crystals of the apo-enzyme belong to space group: P21 with 58.5% solvent and contain four molecules of NaMN AT in the asymmetric subunit, crystals in complex with deamido-NAD+ belong to space group: P212121 with 55.6% solvent and contain six molecules of NaMN AT in the asymmetric subunit, conditions: polyethylene glycol 3350 and 100 mM MgCl2 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.18 NMN
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.18 0.0068
-
 deamido-NAD+ 37°C, pH 7.4 Bacillus subtilis
2.7.7.18 0.043
-
 nicotinamide ribonucleotide 37°C, pH 7.4 Bacillus subtilis
2.7.7.18 0.135
-
 ATP 37°C, pH 7.4 Bacillus subtilis
2.7.7.18 1.7
-
 NAD+ 37°C, pH 7.4 Bacillus subtilis
2.7.7.18 2.3
-
 NMN 37°C, pH 7.4 Bacillus subtilis
2.7.7.18 4.2
-
 diphosphate 37°C, pH 7.4 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.18 36370 49140 gel filtration, sedimentation equlibrium centrifugation Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.18 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.18 recombinant enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.18 0.054
-
 NMN as substrate Bacillus subtilis
2.7.7.18 0.34
-
 NAD+ as substrate Bacillus subtilis
2.7.7.18 2.8
-
 deamido-NAD+ as substrate Bacillus subtilis
2.7.7.18 10.1
-
 ATP as substrate Bacillus subtilis
2.7.7.18 11.1
-
 nicotinate ribonucleotide as substrate Bacillus subtilis
2.7.7.18 11.5
-
 diphosphate as substrate Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.18 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Bacillus subtilis diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + NMN
-
 Bacillus subtilis diphosphate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.18 dimer crystallization studies Bacillus subtilis