Literature summary extracted from
Zhang, H.; Zhou, T.; Kurnasov, O.; Cheek, S.; Grishin, N.V.; Osterman, A.
Crystal structures of E. coli nicotinate mononucleotide adenylyltransferase and its complex with deamido-NAD (2002), Structure, 10, 69-79.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.18 |
overexpression in Escherichia coli BL21 with a 6 x His-tag |
Escherichia coli |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.7.18 |
crystallization of the apo-enzyme to space group: P1 with 44% sovent, and in complex with deamido-NAD+ with 42.4% solvent in space group I222, hanging-drop vapour-diffusion method at 20°C in 100 mM Tris, pH 7, 200 mM NaCl and 800 mM sodium citrate |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.18 |
Escherichia coli |
P0A752 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.7.18 |
recombinant enzyme |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.18 |
ATP + nicotinate ribonucleotide |
i.e. nicotinate mononucleotide |
Escherichia coli |
diphosphate + deamido-NAD+ |
- |
r |
|