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Literature summary extracted from

  • Veitch, D.P.; Gilham, D.; Cornell, R.B.
    The role of histidine residues in the HXGH site of CTP:phosphocholine cytidylyltransferase in CTP binding and catalysis (1998), Eur. J. Biochem., 255, 227-234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15
-
Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 H89A no enzymatic activity Rattus norvegicus
2.7.7.15 H89G no enzymatic activity Rattus norvegicus
2.7.7.15 H89N 100fold decrease in Vmax Rattus norvegicus
2.7.7.15 H89Q no enzymatic activity Rattus norvegicus
2.7.7.15 H92A no enzymatic activity Rattus norvegicus
2.7.7.15 H92G no enzymatic activity Rattus norvegicus
2.7.7.15 H92N reduced Vmax, increased Km Rattus norvegicus
2.7.7.15 H92Q mutation has only little effect on substrate binding and Vmax Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.15 Urea 50% inhibition of wild-type enzyme at 0.75 M, 50% inhibition of H92N mutant enzyme at 0.35 M, 50% inhibition of H92Q mutant enzyme at 0.2 M Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.34
-
CTP wild-type, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 0.37
-
CTP H89N mutant, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 0.48
-
phosphocholine wild-type, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 0.5
-
CTP H92Q mutant, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 0.56
-
phosphocholine wild-type, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 0.63
-
CTP wild-type, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 0.8
-
CTP H92Q mutant, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 0.87
-
phosphocholine H92Q mutant, cytosolic and purified enzyme, 37°C Rattus norvegicus
2.7.7.15 0.98
-
phosphocholine H89N mutant, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 1.73
-
CTP H92N mutant, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 2.4
-
phosphocholine H92N mutant, cytosolic enzyme, 37°C Rattus norvegicus
2.7.7.15 2.65
-
phosphocholine H92N mutant, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 4.2
-
CTP H92N mutant, cytosolic enzyme, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 cytosol
-
Rattus norvegicus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15
-
Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.15 CTP + phosphocholine = diphosphate + CDP-choline catalytic mechanism Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
Rattus norvegicus diphosphate + CDPcholine
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.15 0.5
-
phosphocholine H92N mutant, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 1.63
-
phosphocholine H92Q mutant, purified enzyme, 37°C Rattus norvegicus
2.7.7.15 2.22
-
phosphocholine wild-type, purified enzyme, 37°C Rattus norvegicus