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Literature summary extracted from

  • Tijburg, L.B.M.; Vermeulen, P.S.; van Golde, L.M.G.
    Ethanolamine-phosphate cytidylyltransferase (1992), Methods Enzymol., 209, 258-263.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.14 additional information addition of lipids does not stimulate the activity of cytosolic enzyme Rattus norvegicus
2.7.7.14 Reducing agent activity of purified enzyme is dependent on the presence of DTT Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.7.14 purified enzyme can be stabilized by the addition of 10% v/v glycerol or 2% bovine serum albumin Rattus norvegicus
2.7.7.14 quite stable towards freezing and thawing Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.14 0.053
-
 CTP
-
 Rattus norvegicus
2.7.7.14 0.065
-
 Ethanolamine phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.14 cytosol predominantly Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.14 100000 120000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.14 CTP + ethanolamine phosphate Rattus norvegicus catalyzes a central step in phosphatidylethanolamine synthesis diphosphate + CDP-ethanolamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.14 1162fold to homogeneity Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.14 CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine ordered reaction mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.14 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.14 4.38
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.7.14 -20°C, stable for at least 4 weeks without significant loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.14 CTP + ethanolamine phosphate
-
 Rattus norvegicus diphosphate + CDP-ethanolamine
-
 r
2.7.7.14 CTP + ethanolamine phosphate catalyzes a central step in phosphatidylethanolamine synthesis Rattus norvegicus diphosphate + CDP-ethanolamine
-
 r
2.7.7.14 dCTP + ethanolamine phosphate
-
 Rattus norvegicus diphosphate + dCDP-ethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.14 ? x * 49000-50000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.14 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.14 6
-
 one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 Rattus norvegicus
2.7.7.14 7.8
-
 one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.14 7
-
 limited stability below Rattus norvegicus