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Literature summary extracted from

  • Smoot, J.W.; Serif, G.S.
    Purification of GTP:alpha-D-mannose-1-phosphate guanyltransferase (1985), Eur. J. Biochem., 148, 83-87.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.0004
-
 mannose 1-phosphate pH 7.8, 37°C Sus scrofa
2.7.7.13 0.001
-
 GDP-alpha-D-mannose pH 7.8, 37°C Sus scrofa
2.7.7.13 0.0035
-
 GTP pH 7.8, 37°C Sus scrofa
2.7.7.13 1
-
 GTP pH 7.8, 37°C, cosubstrate diphosphate Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 412000
-
 sucrose velocity sedimentation Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
 GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 blue-Sepharose, DEAE-Sepharose, phenyl-Sepharose, agarose-GTP affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.13 thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 24.1
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.7.13 4°C, stable for a few days, purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 dGTP + alpha-D-mannose 1-phosphate 34% of the activity with GTP Sus scrofa dGDPmannose + diphosphate
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Sus scrofa GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate specific for mannose Sus scrofa GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate no activity with ATP, CTP, TTP, UTP, ADP-alpha-D-glucose, ADP-alpha-D-mannose, CDP-alpha-D-choline, CDP-alpha-D-glucose, CDP-beta-L-fucose, GDP-alpha-D-glucose, dTPP-alpha-D-glucose, IDP-alpha-D-galactose, UDP-alpha-D-glucose, UDP-N-acetyl-alpha-D-glucosamine, UDP-alpha-D-mannose Sus scrofa GDPmannose + diphosphate
-
 r
2.7.7.13 ITP + alpha-D-mannose 1-phosphate 44% of the activity with GTP Sus scrofa IDPmannose + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 6 7.5
-
 Sus scrofa