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Literature summary extracted from

  • Lee, L.J.; Kimura, A.; Tochikura, T.
    Purification and properties of UDP-glucose (UDP-galactose) pyrophosphorylase from Bifidobacterium bifidum (1979), J. Biochem., 86, 923-928.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.9 3-phosphoglycerate slight activation Bifidobacterium bifidum
2.7.7.9 D-erythrose 4-phosphate slight activation Bifidobacterium bifidum
2.7.7.9 D-fructose 6-phosphate slight activation Bifidobacterium bifidum
2.7.7.10 3-phosphoglycerate slight activation Bifidobacterium bifidum
2.7.7.10 D-erythrose 4-phosphate slight activation Bifidobacterium bifidum
2.7.7.10 D-fructose 6-phosphate slight activation Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum
2.7.7.9 alpha-D-glucose 1-phosphate UDP-glucose pyrophosphorolysis Bifidobacterium bifidum
2.7.7.9 D-galactose 6-phosphate UDP-glucose pyrophosphorolysis Bifidobacterium bifidum
2.7.7.9 UDP-galactose UDP-glucose pyrophosphorolysis Bifidobacterium bifidum
2.7.7.9 UDP-glucose UDP-galactose pyrophosphorolysis or UDP-glucose synthesis Bifidobacterium bifidum
2.7.7.9 UDP-xylose
-
 Bifidobacterium bifidum
2.7.7.9 UTP UDP-glucose pyrophosphorolysis Bifidobacterium bifidum
2.7.7.10 additional information no inhibitors of UDP-galactose pyrophosphorolysis are alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate or galactose 6-phosphate Bifidobacterium bifidum
2.7.7.10 UDP-galactose UDP-glucose pyrophosphorolysis or UDP-galactose synthesis Bifidobacterium bifidum
2.7.7.10 UDP-glucose UDP-galactose pyrophosphorolysis Bifidobacterium bifidum
2.7.7.10 UDP-xylose UDP-galactose or UDP-glucose synthesis Bifidobacterium bifidum
2.7.7.10 UTP UDP-galactose pyrophosphorolysis Bifidobacterium bifidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ requirement Bifidobacterium bifidum
2.7.7.10 Mg2+ requirement Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 51000
-
 4 * 51000, SDS-PAGE Bifidobacterium bifidum
2.7.7.9 200000
-
 gel filtration Bifidobacterium bifidum
2.7.7.10 51000
-
 4 * 51000, SDS-PAGE Bifidobacterium bifidum
2.7.7.10 200000
-
 gel filtration Bifidobacterium bifidum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Bifidobacterium bifidum
-
-
-
2.7.7.10 Bifidobacterium bifidum
-
 glucose-grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9
-
 Bifidobacterium bifidum
2.7.7.10
-
 Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 15.2
-
 purified enzyme Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 diphosphate + UDP-xylose reaction at 10% the rate of UDP-glucose or UDP-galactose pyrophosphorolysis Bifidobacterium bifidum UTP + D-xylose 1-phosphate
-
 r
2.7.7.9 additional information UDP-glucose and UDP-galactose pyrophosphorolysis activity in Bifidobacterium bifidum are catalyzed by a single enzyme protein Bifidobacterium bifidum ?
-
 ?
2.7.7.9 UTP + alpha-D-galactose 1-phosphate at the same rate as UDP-glucose Bifidobacterium bifidum diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate reaction at the same rate as UDP-galactose Bifidobacterium bifidum diphosphate + UDP-glucose
-
 r
2.7.7.10 diphosphate + UDP-xylose at 10% the rate of UDP-glucose or UDP-galactose Bifidobacterium bifidum ?
-
 r
2.7.7.10 additional information no activity in the reverse reaction with UDP-N-acetylglucosamine, ADP-glucose, CDP-glucose, GDP-mannose or TDP-glucose Bifidobacterium bifidum ?
-
 ?
2.7.7.10 UTP + alpha-D-galactose 1-phosphate reverse reaction at the same rate as UDP-glucose Bifidobacterium bifidum diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-glucose 1-phosphate reverse reaction at the same rate as UDP-galactose Bifidobacterium bifidum diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 tetramer 4 * 51000, SDS-PAGE Bifidobacterium bifidum
2.7.7.10 tetramer 4 * 51000, SDS-PAGE Bifidobacterium bifidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 37
-
 assay at Bifidobacterium bifidum
2.7.7.10 37
-
 assay at Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 5.5 7 broad Bifidobacterium bifidum
2.7.7.10 5.5 7 broad Bifidobacterium bifidum