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Literature summary extracted from

  • Raffaelli, N.; Lorenzi, T.; Emanuelli, M.; Amici, A.; Ruggieri, S.; Magni, G.
    Nicotinamide-mononucleotide adenylyltransferase from Sulfolobus solfataricus (2001), Methods Enzymol., 331, 281-292.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.1 SO42-
-
 Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
2.7.7.1 chaotropic anions are strong destabilizers of the enzyme Saccharolobus solfataricus
2.7.7.1 dithiothreitol has no effect up to 50°C but destabilizes the enzyme at higher temperatures Saccharolobus solfataricus
2.7.7.1 increasing ionic strenght of NaCl and KCl stabilizes the thermal stability of the enzyme Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 KCl
-
 Saccharolobus solfataricus
2.7.7.1 Mg2+
-
 Saccharolobus solfataricus
2.7.7.1 Na+
-
 Saccharolobus solfataricus
2.7.7.1 NH4+
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 18600
-
 x * 18600, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 8.8
-
 purification step: TSK phenyl-5PW Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharolobus solfataricus diphosphate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 ? x * 18600, SDS-PAGE Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.1 37 97 activity shows a continuous increase up to 97°C Saccharolobus solfataricus