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Literature summary extracted from

  • Saridakis, V.; Christendat, D.; Kimber, M.S.; Dharamsi, A.; Edwards, A.M.; Pai, E.F.
    Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes (2001), J. Biol. Chem., 276, 7225-7232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 expression in Escherichia coli BL21 Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.1 hanging drop vapor diffusion method, in 100 mM HEPES, pH 7.5 at 20°C, crystals of native enzyme were grown in 1.5 M Li2SO4, crystals of H19A enzyme were grown in 1.6 M Li2SO4, space group: P6322 Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.1 H19A loss of activity Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Methanothermobacter thermautotrophicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Methanothermobacter thermautotrophicus diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Methanothermobacter thermautotrophicus diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 hexamer x-ray crystallography Methanothermobacter thermautotrophicus