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Literature summary extracted from

  • Magni, G.; Natalini, P.; Santarelli, I.; Vita, A.; Raffaelli, N.; Ruggieri, S.
    NAD pyrophosphorylase from yeast chromatin. Purification and properties (1987), Basic Appl. Histochem., 31, 255-271.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.000019
-
 NMN
-
 Saccharomyces cerevisiae
2.7.7.1 0.11
-
 ATP
-
 Saccharomyces cerevisiae
2.7.7.1 0.147 0.2 nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae
2.7.7.1 5
-
 nicotinate ribonucleotide
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 50000
-
 alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae
2.7.7.1 200000
-
 gel filtration Saccharomyces cerevisiae
2.7.7.1 200000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Saccharomyces cerevisiae
-
 baker's yeast
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.1 glycoprotein possibly ADP-ribosylated Saccharomyces cerevisiae
2.7.7.1 glycoprotein 2% carbohydrates Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 tetramer alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae