Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Natalini, P.; Ruggieri, S.; Raffaelli, N.; Magni, G.
    Nicotinamide mononucleotide adenylyltransferase. Molecular and enzymatic properties of the homogeneous enzyme from bakers yeast (1986), Biochemistry, 25, 3725-3729.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.1 bovine serum albumin stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.1 diphosphate product inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.000019
-
 NMN 37°C, pH 7.6 Saccharomyces cerevisiae
2.7.7.1 0.11
-
 ATP 37°C, pH 7.6 Saccharomyces cerevisiae
2.7.7.1 0.147 0.2 nicotinamide ribonucleotide 37°C, pH 7.6 Saccharomyces cerevisiae
2.7.7.1 5
-
 nicotinate ribonucleotide 37°C, pH 7.6 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 additional information
-
 amino acid composition Saccharomyces cerevisiae
2.7.7.1 50000
-
 alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae
2.7.7.1 200000
-
 gel filtration Saccharomyces cerevisiae
2.7.7.1 200000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Saccharomyces cerevisiae
-
 baker's yeast
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.1 glycoprotein 2% carbohydrates Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 2.8
-
 purification step: Matrix Gel Green A Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.7.1 -20°C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month Saccharomyces cerevisiae
2.7.7.1 4°C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide best substrate Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide less efficient Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 tetramer alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 7.8 8.4
-
 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.1 Saccharomyces cerevisiae in the presence of 4 M urea, multiple pIs in its absence at pH 4.2, 4.9 and 6.2
-
 6.2