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Literature summary extracted from

  • Emanuelli, M.; Natalini, P.; Raffaelli, N.; Ruggieri, S.; Vita, A.; Magni, G.
    NAD biosynthesis in human placenta: purification and characterization of homogeneous NMN adenylyltransferase (1992), Arch. Biochem. Biophys., 298, 29-34.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.1 dithiothreitol and EDTA stabilize during purification Homo sapiens
2.7.7.1 extremely labile in crude extracts, even in the presence of PMSF and sodium metabisulfite, EDTA or DTT does not stabilize Homo sapiens
2.7.7.1 very sensitive to freeze-thawing, 50% glycerol stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.1 ADPribose reverse reaction Homo sapiens
2.7.7.1 beta-NMNH
-
 Homo sapiens
2.7.7.1 Cd2+
-
 Homo sapiens
2.7.7.1 Cr3+
-
 Homo sapiens
2.7.7.1 Cu2+ strong Homo sapiens
2.7.7.1 Fe3+
-
 Homo sapiens
2.7.7.1 Hg2+
-
 Homo sapiens
2.7.7.1 additional information no inhibition by pyrimidine or purine bases, oxy- and deoxynucleosides, cAMP, halogenated and methylated nucleobases, oxy- and deoxyribose, oxy- and deoxyribose 5-phosphate, D-alpha-glucose, D-alpha-glucose 1-phosphate, D-alpha-glucose 6-phosphate, D-alpha-glucose 1,6-diphosphate, D-alpha-fructose, D-alpha-fructose 1,6-diphosphate, alpha-nicotinamide ribonucleotide; product inhibition study Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.023
-
 ATP 37°C, pH 7.6 Homo sapiens
2.7.7.1 0.038
-
 nicotinamide ribonucleotide 37°C, pH 7.6 Homo sapiens
2.7.7.1 0.067 0.069 NAD+ 37°C, pH 7.6 Homo sapiens
2.7.7.1 0.125
-
 diphosphate 37°C, pH 7.6 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.1 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Mg2+ requirement Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 33000
-
 alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol Homo sapiens
2.7.7.1 132000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Homo sapiens key reaction of pyridine nucleotide metabolism diphosphate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.1 ATP + nicotinamide ribonucleotide = diphosphate + NAD+ mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.1 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 9.1 10.2
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.7.1 -80°C, 1 mM DTT, 0.5 mM EDTA, 2 weeks Homo sapiens
2.7.7.1 4°C, in 50 mM potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 1 mM PMSF, several months Homo sapiens
2.7.7.1 high KCl concentrations stabilize during storage Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide best substrate Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide strongly stereospecific: beta-, not alpha-anomer of nicotinamide ribonucleotide Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide key reaction of pyridine nucleotide metabolism Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide less efficient Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 tetramer alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 6 9 plateau Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.1 Homo sapiens isoelectric focusing, LKB 8100 ampholine column filled with a linear gradient of glycerol containing 1% ampholine carrier ampholytes 4.8 4.7