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Literature summary extracted from

  • Dahmen, W.; Webb, B.; Preiss, J.
    The deamido-diphosphopyridine nucleotide and diphosphopyridine nucleotide pyrophosphorylases of Escherichia coli and yeast (1967), Arch. Biochem. Biophys., 120, 440-450.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.1 NH4Cl activation, can substitute for KCl Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.1 NAD+ deamide-NAD+ as substrate Escherichia coli
2.7.7.18 NAD+ deamido-NAD+ as substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.0045
-
 deamido-NAD+ 37°C, pH 7.5 Escherichia coli
2.7.7.1 0.029
-
 deamido-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.06
-
 ATP 37°C, pH 7.5, + nicotinate ribonucleotide Saccharomyces cerevisiae
2.7.7.1 0.067 0.069 NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.08
-
 nicotinate ribonucleotide 37°C, pH 7.5 Escherichia coli
2.7.7.1 0.13
-
 nicotinate ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.147 0.2 nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae
2.7.7.1 0.147 0.2 nicotinamide ribonucleotide 37°C, pH 7.5 Escherichia coli
2.7.7.1 0.27
-
 3-acetylpyridine-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.37
-
 NAD+ 37°C, pH 7.5 Escherichia coli
2.7.7.1 0.4
-
 nicotinamide ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.5 0.52 ATP 37°C, pH 7.5 Escherichia coli
2.7.7.1 0.65
-
 diphosphate 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 0.74
-
 3-pyridinealdehyde-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.1 1.1
-
 diphosphate 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.0045
-
 deamido-NAD+ 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.029
-
 deamido-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.06
-
 ATP 37°C, pH 7.5, + nicotinate ribonucleotide Saccharomyces cerevisiae
2.7.7.18 0.069
-
 NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.08
-
 nicotinate ribonucleotide 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.13
-
 nicotinate ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.2
-
 nicotinamide ribonucleotide 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.27
-
 3-acetylpyridine-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.37
-
 NAD+ 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.4
-
 nicotinamide ribonucleotide 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.5
-
 ATP 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.65
-
 diphosphate 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 0.74
-
 3-pyridinealdehyde-NAD+ 37°C, pH 7.5 Saccharomyces cerevisiae
2.7.7.18 1.1
-
 diphosphate 37°C, pH 7.5 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 KCl activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis Escherichia coli
2.7.7.1 Mg2+ requirement Escherichia coli
2.7.7.1 Mg2+ requirement Saccharomyces cerevisiae
2.7.7.18 KCl activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis Escherichia coli
2.7.7.18 Mg2+ requirement Escherichia coli
2.7.7.18 Mg2+ requirement Saccharomyces cerevisiae
2.7.7.18 NH4Cl activation, can substitute for KCl Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Escherichia coli
-
 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide Escherichia coli B / ATCC 11303
-
 diphosphate + NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide Escherichia coli
-
 diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + deamido-NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Escherichia coli
-
 B
-
2.7.7.1 Escherichia coli B / ATCC 11303
-
 B
-
2.7.7.1 Saccharomyces cerevisiae
-
 brewer's yeast
-
2.7.7.18 Escherichia coli
-
-
-
2.7.7.18 Saccharomyces cerevisiae
-
 brewer's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1 partial Escherichia coli
2.7.7.1 partial Saccharomyces cerevisiae
2.7.7.18 partial Escherichia coli
2.7.7.18 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 0.0038
-
 NAD+-synthesis Escherichia coli
2.7.7.1 0.004
-
 NAD+ as substrate Escherichia coli
2.7.7.1 0.024
-
 deamido-NAD+ as substrate Escherichia coli
2.7.7.1 0.068
-
 deamido-NAD+-synthesis Escherichia coli
2.7.7.1 3.3
-
 NAD+-synthesis Saccharomyces cerevisiae
2.7.7.1 3.85
-
 deamido-NAD+ as substrate Saccharomyces cerevisiae
2.7.7.1 5
-
 NAD+ as substrate, yeast Saccharomyces cerevisiae
2.7.7.1 7.3
-
 deamido-NAD+-synthesis Saccharomyces cerevisiae
2.7.7.18 0.0003
-
 3-acetyl-pyridine-NAD+ as substrate Escherichia coli
2.7.7.18 0.0008
-
 3-pyridinealdehyde-NAD+ as substrate Escherichia coli
2.7.7.18 0.004
-
 NAD+ as substrate Escherichia coli
2.7.7.18 0.024
-
 deamido-NAD+ as substrate Escherichia coli
2.7.7.18 1.42
-
 3-pyridinealdehyde-NAD+ as substrate Saccharomyces cerevisiae
2.7.7.18 3.82
-
 3-acetyl-pyridine-NAD+ as substrate Saccharomyces cerevisiae
2.7.7.18 3.85
-
 deamido-NAD+ as substrate Saccharomyces cerevisiae
2.7.7.18 5
-
 NAD+ as substrate Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.7.18 0-3°C, 22% loss of activity within 31 days Saccharomyces cerevisiae
2.7.7.18 0-3°C, 31 days stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + 3-acetylpyridine-NAD+ poor substrate Escherichia coli ?
-
 ?
2.7.7.1 ATP + 3-acetylpyridine-NAD+ reaction at 76% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
2.7.7.1 ATP + 3-pyridinealdehyde-NAD+ poor substrate Escherichia coli ?
-
 ?
2.7.7.1 ATP + 3-pyridinealdehyde-NAD+ reaction at 28% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Escherichia coli diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide best substrate Escherichia coli diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide best substrate Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Escherichia coli diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Escherichia coli diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide best substrate Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Escherichia coli B / ATCC 11303 diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide best substrate Escherichia coli diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Escherichia coli diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide reaction at 77% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
2.7.7.1 deoxy-ATP + nicotinamide ribonucleotide
-
 Escherichia coli ?
-
 ?
2.7.7.1 deoxy-ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.7.1 deoxy-ATP + nicotinate ribonucleotide reaction at 18% the rate of ATP Escherichia coli ?
-
 ?
2.7.7.1 deoxy-ATP + nicotinate ribonucleotide reaction at 30% the rate of ATP Saccharomyces cerevisiae ?
-
 ?
2.7.7.1 nicotinate mononucleotide + ATP
-
 Escherichia coli nicotinate adenine dinucleotide + diphosphate
-
 ?
2.7.7.1 nicotinate mononucleotide + ATP
-
 Saccharomyces cerevisiae nicotinate adenine dinucleotide + diphosphate
-
 ?
2.7.7.18 ATP + 3-acetyl-pyridine-NAD+ poor substrate Escherichia coli ?
-
 ?
2.7.7.18 ATP + 3-acetyl-pyridine-NAD+ reaction at 76% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
2.7.7.18 ATP + 3-pyridinealdehyde-NAD+ poor substrate Escherichia coli ?
-
 ?
2.7.7.18 ATP + 3-pyridinealdehyde-NAD+ reaction at 28% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
 ?
2.7.7.18 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide, reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli diphosphate + NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide
-
 Escherichia coli diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide best substrate Escherichia coli diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
2.7.7.18 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Escherichia coli diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
2.7.7.18 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
2.7.7.18 ATP + nicotinate ribonucleotide reaction at 77% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
2.7.7.18 deoxy-ATP + nicotinamide ribonucleotide
-
 Escherichia coli ?
-
 ?
2.7.7.18 deoxy-ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.7.18 deoxy-ATP + nicotinate ribonucleotide
-
 Escherichia coli ?
-
 ?
2.7.7.18 deoxy-ATP + nicotinate ribonucleotide
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.7.18 NAD+ + diphosphate
-
 Saccharomyces cerevisiae ATP + nicotinamide nucleotide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 37
-
 assay at Escherichia coli
2.7.7.1 37
-
 assay at Saccharomyces cerevisiae
2.7.7.18 37
-
 assay at Escherichia coli
2.7.7.18 37
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.1 55
-
 5 min 54% loss of activity Escherichia coli
2.7.7.1 60
-
 5 min 39% loss of activity Saccharomyces cerevisiae
2.7.7.1 60
-
 5 min 95% loss of activity Escherichia coli
2.7.7.1 65
-
 5 min 65% loss of activity Saccharomyces cerevisiae
2.7.7.18 55
-
 5 min 54% loss of activity Escherichia coli
2.7.7.18 60
-
 5 min 39% loss of activity Saccharomyces cerevisiae
2.7.7.18 60
-
 5 min 95% loss of activity Escherichia coli
2.7.7.18 65
-
 5 min 65% loss of activity Saccharomyces cerevisiae