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Literature summary extracted from

  • Peterson, J.W.; Gubler, C.J.; Kuby, S.A.
    Partial purification and properties of thiamine pyrophosphokinase from pig brain (1975), Biochim. Biophys. Acta, 397, 377-394.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.2 Butylthiamine competitive with respect to thiamine Sus scrofa
2.7.6.2 ethylthiamine competitive with respect to thiamine Sus scrofa
2.7.6.2 oxythiamine competitive with respect to thiamine Sus scrofa
2.7.6.2 pyrithiamine competitive with respect to thiamine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.2 0.0041
-
 ATP pH 7.8, 37°C Sus scrofa
2.7.6.2 0.059
-
 ATP pH 7.8, 37°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.2 Mg2+ for optimal activity the ratio of magnesium to ATP must be fixed at 0.6. The Mg(ATP)26- species might by the actual diphosphoryl donor Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.2 partial Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.2 ATP + thiamine = AMP + thiamine diphosphate partial ping pong mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.2 brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.2 0.00165
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 ATP + thiamine
-
 Sus scrofa AMP + thiamine diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.2 8.3 9.3 in 0.028 M phosphate/glycylglycine buffer Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.2 6.8 9.3 pH 6.8: about 65% of maximal activity, pH 8.3-9.3: optimum Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.2 Sus scrofa isoelectric focusing
-
 4.2