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Literature summary extracted from

  • Mitsuda, H.; Takii, Y.; Iwami, K.; Yasumoto, K.; Nakajima, K.
    Enzymatic formation of thiamine pyrophosphate in plants (1979), Methods Enzymol., 62, 107-111.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.2 diphosphate 10 mM, complete inhibition Petroselinum crispum
2.7.6.2 iodoacetamide 10 mM Petroselinum crispum
2.7.6.2 KCN little effect Petroselinum crispum
2.7.6.2 molybdate little effect Petroselinum crispum
2.7.6.2 NaF little effect Petroselinum crispum
2.7.6.2 NEM
-
 Petroselinum crispum
2.7.6.2 PCMB 0.1 mM, complete inhibition, completely restored by 10 mM dithiothreitol Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.2 0.00015
-
 thiamine
-
 Petroselinum crispum
2.7.6.2 0.8
-
 ATP
-
 Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.2 30000
-
 gel filtration Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Petroselinum crispum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.2 leaf
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.2 0.000722
-
-
 Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 ATP + thiamine
-
 Petroselinum crispum AMP + thiamine diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.2 8 9 in Tris buffer Petroselinum crispum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.2 Petroselinum crispum isoelectric focusing
-
 4.5