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Literature summary extracted from

  • Krath, B.N.; Hove-Jensen, B.
    Organellar and cytosolic localization of four phosphoribosyl diphosphate synthase isozymes in spinach (1999), Plant Physiol., 119, 497-506.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate isoenzymes PRS1 and PRS2 require phosphate for activity, isoenzymes PRS3 and PRS4 are phosphate independent Spinacia oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression of isoenzymes PRS1, PRS2, PRS3 and PRS4 in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP inhibits isoenzymes PRS1 and PRS2 efficiently, but not isoenzymes PRS3 and PRS4 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.1 chloroplast
-
Spinacia oleracea 9507
-
2.7.6.1 mitochondrion
-
Spinacia oleracea 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Spinacia oleracea
-
AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Spinacia oleracea
-
spinach
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 0.001
-
mitochondria preparation Spinacia oleracea
2.7.6.1 0.004
-
chloroplast preparation Spinacia oleracea
2.7.6.1 0.014
-
isoenzyme PRS1, without phosphate and ADP Spinacia oleracea
2.7.6.1 0.018
-
isoenzyme PRS2, in the presence of phosphate 50 mM, and ADP 1 mM Spinacia oleracea
2.7.6.1 0.025
-
isoenzyme PRS2, without phosphate and ADP Spinacia oleracea
2.7.6.1 0.072
-
isoenzyme PRS4, in the presence of phosphate 50 mM, and ADP 1 mM Spinacia oleracea
2.7.6.1 0.074
-
isoenzyme PRS1, in the presence of phosphate 50 mM, and ADP 1 mM Spinacia oleracea
2.7.6.1 0.08
-
isoenzyme PRS4, in the presence of phosphate 50 mM, without ADP Spinacia oleracea
2.7.6.1 0.089
-
isoenzyme PRS4, without phosphate and ADP Spinacia oleracea
2.7.6.1 0.099
-
isoenzyme PRS4, in the presence of ADP 1 mM, without phosphate Spinacia oleracea
2.7.6.1 0.14
-
isoenzyme PRS2, in the presence of phosphate 50 mM, without ADP Spinacia oleracea
2.7.6.1 0.191
-
isoenzyme PRS1, in the presence of phosphate 50 mM, without ADP Spinacia oleracea
2.7.6.1 0.88
-
isoenzyme PRS3, in the presence of phosphate 50 mM, and ADP 1 mM Spinacia oleracea
2.7.6.1 0.884
-
isoenzyme PRS3, in the presence of ADP 1 mM, without phosphate Spinacia oleracea
2.7.6.1 1.06
-
isoenzyme PRS3, in the presence of phosphate 50 mM, without ADP Spinacia oleracea
2.7.6.1 1.26
-
isoenzyme PRS3, without phosphate and ADP Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 30
-
assay at Spinacia oleracea