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Literature summary extracted from

  • Gallois, R.; Prevot, J.C.; Clement, A.; Jacob, J.L.
    Purification and characterization of phosphoribosylpyrophosphate synthetase from rubber tree latex (1997), Plant Physiol., 115, 847-852.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 DTT requirement Hevea brasiliensis
2.7.6.1 additional information no other sulfhydryl group protector or antioxidant can effectively replace DTT Hevea brasiliensis
2.7.6.1 phosphate activation at 1 mM of D-ribose 5-phosphate, maximal activity from 10 mM of D-ribose 5-phosphate Hevea brasiliensis
2.7.6.1 sulfate no activity observed Hevea brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 5-phospho-alpha-D-ribose 1-diphosphate
-
 Hevea brasiliensis
2.7.6.1 adenosine no inhibition Hevea brasiliensis
2.7.6.1 ADP no inhibition Hevea brasiliensis
2.7.6.1 AMP no inhibition Hevea brasiliensis
2.7.6.1 ATP no inhibition Hevea brasiliensis
2.7.6.1 D-fructose 1,6-diphosphate no inhibition Hevea brasiliensis
2.7.6.1 GDP no inhibition Hevea brasiliensis
2.7.6.1 glyceraldehyde 3-phosphate no inhibition Hevea brasiliensis
2.7.6.1 GMP no inhibition Hevea brasiliensis
2.7.6.1 GTP no inhibition Hevea brasiliensis
2.7.6.1 guanosine no inhibition Hevea brasiliensis
2.7.6.1 L-histidine no inhibition Hevea brasiliensis
2.7.6.1 L-tryptophan no inhibition Hevea brasiliensis
2.7.6.1 Mn2+ complete inhibition above 0.4 mM Hevea brasiliensis
2.7.6.1 additional information D-glucose-6-phosphate have no effects on the enzyme activity; dihydroxyacetone phosphate, D-glucose-1-phosphate, fructose-6-phosphate, ribose, sucrose, glutamine, glutamic acid, aspartic acid, alanine, alpha-ketoglutarate, pyruvate, citrate, malate, fumarate, succinate, cytidine, uridine, adenine, orotate, guanine, cytosine and xanthine have no effects on the enzyme activity Hevea brasiliensis
2.7.6.1 phosphate inhibition at low concentrations of D-ribose 5-phosphate Hevea brasiliensis
2.7.6.1 UDP no inhibition Hevea brasiliensis
2.7.6.1 UMP no inhibition Hevea brasiliensis
2.7.6.1 UTP no inhibition Hevea brasiliensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.04
-
 D-ribose 5-phosphate pH 7.5, 37ºC Hevea brasiliensis
2.7.6.1 0.2
-
 ATP pH 7.5, 37ºC Hevea brasiliensis
2.7.6.1 1.5
-
 Mg2+ pH 7.5, 37ºC Hevea brasiliensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.1 cytosol also known as latex in specialized cells Hevea brasiliensis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Co2+ no activity observed Hevea brasiliensis
2.7.6.1 Mg2+ requirement Hevea brasiliensis
2.7.6.1 Mn2+ no activity observed Hevea brasiliensis
2.7.6.1 additional information K+, Na+, Fe2+ Cu2+ and Ca2+ have no notable effects on the enzyme activity Hevea brasiliensis
2.7.6.1 Zn2+ no activity observed Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 57000
-
 x * 57000, SDS-PAGE Hevea brasiliensis
2.7.6.1 200000
-
 gel filtration Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Hevea brasiliensis
-
 rubber tree
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 ammonium sulfate fractionation, chromatography on Sephacryl S300 HR and on Protein-Pak DEAE 40 HR and affinity chromatography Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 laticifer
-
 Hevea brasiliensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Hevea brasiliensis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 CTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 GTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 ITP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis IMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 57000, SDS-PAGE Hevea brasiliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 30
-
 assay at Hevea brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.3
-
-
 Hevea brasiliensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.02
-
 Mn2+ pH 7.5, 37ºC Hevea brasiliensis
2.7.6.1 0.03
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.5, 37ºC Hevea brasiliensis