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Literature summary extracted from

  • Tatibana, M.; Kita, K.; Taira, M.; Ishijima, S.; Sonoda, T.; Ishizuka, T.; Iizasa, T.; Ahmad, I.
    Mammalian phosphoribosyl-pyrophosphate synthetase (1995), Adv. Enzyme Regul., 35, 229-249.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate specific and absolute requirement Homo sapiens
2.7.6.1 phosphate specific and absolute requirement Rattus norvegicus
2.7.6.1 sulfate can partially replace phosphate in activation Homo sapiens
2.7.6.1 sulfate can partially replace phosphate in activation Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression of isoenzymes PRSI and PRSII in Escherichia coli Homo sapiens
2.7.6.1 expression of isoenzymes PRSI and PRSII in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP
-
 Homo sapiens
2.7.6.1 ADP one of the most effective Rattus norvegicus
2.7.6.1 AMP
-
 Homo sapiens
2.7.6.1 AMP one of the most effective Rattus norvegicus
2.7.6.1 CTP
-
 Homo sapiens
2.7.6.1 CTP weak inhibition at 1 mM Rattus norvegicus
2.7.6.1 GDP
-
 Homo sapiens
2.7.6.1 GDP one of the most effective Rattus norvegicus
2.7.6.1 GTP
-
 Homo sapiens
2.7.6.1 GTP weak inhibition at 1 mM Rattus norvegicus
2.7.6.1 PRPP synthetase-associated proteins inhibit catalytic and perhaps regulatory functions of the enzyme Homo sapiens
2.7.6.1 PRPP synthetase-associated proteins inhibit catalytic and perhaps regulatory functions of the enzyme Rattus norvegicus
2.7.6.1 UDP 10%-16% inhibition at 1 mM Homo sapiens
2.7.6.1 UDP 10%-16% inhibition at 1 mM Rattus norvegicus
2.7.6.1 UMP 10%-18% inhibition at 1 mM Homo sapiens
2.7.6.1 UMP 10%-18% inhibition at 1 mM Rattus norvegicus
2.7.6.1 UTP weak inhibition at 1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.04
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 0.044
-
 ATP pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 0.06
-
 ATP pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
2.7.6.1 0.073
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.1 brush border intestinal mucosa Rattus norvegicus 5903
-
2.7.6.1 cytosol 60% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation Rattus norvegicus 5829
-
2.7.6.1 microsome 30% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
-
 Homo sapiens
2.7.6.1 additional information
-
 the catalytic subunit complexes with the PRPP synthetase-associated protein of 39000 Da, PAP39, to form highly aggregated forms Rattus norvegicus
2.7.6.1 34000
-
 x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII Rattus norvegicus
2.7.6.1 39000
-
 x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII Rattus norvegicus
2.7.6.1 41000
-
 x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII Rattus norvegicus
2.7.6.1 68000
-
 x * 68000, gel filtration in the presence of MgCl2 1 M Rattus norvegicus
2.7.6.1 1000000
-
-
 Homo sapiens
2.7.6.1 1000000
-
 chromatography on DEAE-Toyopearl Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Homo sapiens the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Homo sapiens
-
-
-
2.7.6.1 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 adipose tissue isoenzymes PRSI and PRSII Rattus norvegicus
-
2.7.6.1 adrenal gland isoenzyme PRSI Rattus norvegicus
-
2.7.6.1 brain isoenzyme PRSI Rattus norvegicus
-
2.7.6.1 erythrocyte
-
 Homo sapiens
-
2.7.6.1 glioblastoma cell cell line Homo sapiens
-
2.7.6.1 liver
-
 Rattus norvegicus
-
2.7.6.1 lung isoenzyme PRSII Rattus norvegicus
-
2.7.6.1 spleen isoenzyme PRSII Rattus norvegicus
-
2.7.6.1 testis isoenzymes PRSI and PRSII Rattus norvegicus
-
2.7.6.1 thymus isoenzymes PRSI and PRSII Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 additional information
-
-
 Homo sapiens
2.7.6.1 additional information
-
 33400 U/mg and 46200 U/mg for PRSI and PRSII purified isoenzymes, respectively Rattus norvegicus
2.7.6.1 additional information
-
 purified enzyme has an activity of 7280 U/mg Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ?
-
 Homo sapiens
2.7.6.1 ? x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII Rattus norvegicus
2.7.6.1 ? x * 68000, gel filtration in the presence of MgCl2 1 M Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 49
-
-
 Homo sapiens
2.7.6.1 49
-
 half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.1 Rattus norvegicus isoenzyme rPRSII 4.4 4.3
2.7.6.1 Rattus norvegicus isoenzyme rPRSI
-
 4.4