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Literature summary extracted from

  • Nosal, J.M.; Switzer, R.L.; Becker, M.A.
    Overexpression, purification, and characterization of recombinant human 5-phosphoribosyl-1-pyrophosphate synthetase isozymes I and II (1993), J. Biol. Chem., 268, 10168-10175.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate requirement Homo sapiens
2.7.6.1 phosphate specific and absolute requirement Homo sapiens
2.7.6.1 phosphate no inhibition up to 75 mM Homo sapiens
2.7.6.1 sulfate can partially replace phosphate in activation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression of isoenzymes PRSI and PRSII in Escherichia coli Homo sapiens

General Stability

EC Number General Stability Organism
2.7.6.1 dilution of highly purified isoenzyme PRSII to 0.1 mg/ml lacking Mg2+ and ATP results in immediate loss of 65% activity and 88% activity after 3 h at 4ºC. Dilution of isoenzyme PRSI to 0.1 mg/ml lacking Mg2+ and ATP results in loss of 10% activity after 3h at 4ºC Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 2,3-diphosphoglycerate at 1 mM, inhibition of isoenzyme PRSI and PRSII, at 1-10 mM, stimulation of isoenzyme PRSI Homo sapiens
2.7.6.1 ADP the most effective Homo sapiens
2.7.6.1 Ca2+
-
Homo sapiens
2.7.6.1 CDP no effect at 1 mM Homo sapiens
2.7.6.1 CTP no effect at 1 mM Homo sapiens
2.7.6.1 GDP
-
Homo sapiens
2.7.6.1 GMP no effect at 1 mM Homo sapiens
2.7.6.1 UDP no effect at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.021
-
ATP pH 7.5, 37ºC, isoenzyme PRSI Homo sapiens
2.7.6.1 0.021
-
ATP ATP in form of MgATP2- Homo sapiens
2.7.6.1 0.052
-
D-ribose 5-phosphate pH 7.5, 37ºC, isoenzyme PRSI Homo sapiens
2.7.6.1 0.07
-
Mg2+ pH 7.5, 37ºC, isoenzyme PRSI Homo sapiens
2.7.6.1 0.083
-
D-ribose 5-phosphate pH 7.5, 37ºC, isoenzyme PRSII Homo sapiens
2.7.6.1 0.11
-
Mg2+ pH 7.5, 37ºC, isoenzyme PRSII Homo sapiens
2.7.6.1 0.7
-
phosphate pH 7.5, 37ºC, isoenzyme PRSI Homo sapiens
2.7.6.1 2.1
-
phosphate pH 7.5, 37ºC, isoenzyme PRSII Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ requirement Homo sapiens
2.7.6.1 Mg2+ the most effective Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
-
Homo sapiens
2.7.6.1 1000000
-
gel filtration, isoenzymes PRSI and PRSII Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Homo sapiens the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 streptomycin precipitation and ammonium sulfate fractionation for isoenzyme PRSI and polyethylene glycol, acid and ammonium sulfate precipitation for isoenzyme PRSII Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 25
-
isoenzyme PRSI Homo sapiens
2.7.6.1 35.7
-
isoenzyme PRSII Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 55
-
isoenzyme PRSII is more thermolabile than PRSI. After 10 min, isoenzyme PRSI loses about 30% of activity, isoenzyme PRSII, about 85% of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.4
-
isoenzyme PRSII Homo sapiens
2.7.6.1 7.5
-
at 40-50 mM phosphate, isoenzymes PRSI and PRSII Homo sapiens
2.7.6.1 8.8
-
isoenzyme PRSI Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.1 6.5 9.3 At pH 6.5, about 25% and 50% of maximum activity for isoenzymes PRSI and PRSII, respectively. At pH 9.3, about 40% and 60% of maximum activity for isoenzymes PRSII and PRSI, respectively Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.6.1 7.4
-
85% of maximal activity, isoenzyme PRSI Homo sapiens
2.7.6.1 8.8
-
41% of maximal activity, isoenzyme PRSII Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.1 Homo sapiens two-dimensional protein electrophoresis, isoenzyme PRSI
-
5.5
2.7.6.1 Homo sapiens two-dimensional protein electrophoresis, isoenzyme PRSII
-
5.7