EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | phosphate | requirement | Homo sapiens | |
2.7.6.1 | phosphate | specific and absolute requirement | Homo sapiens | |
2.7.6.1 | phosphate | no inhibition up to 75 mM | Homo sapiens | |
2.7.6.1 | sulfate | can partially replace phosphate in activation | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.6.1 | expression of isoenzymes PRSI and PRSII in Escherichia coli | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
2.7.6.1 | dilution of highly purified isoenzyme PRSII to 0.1 mg/ml lacking Mg2+ and ATP results in immediate loss of 65% activity and 88% activity after 3 h at 4ºC. Dilution of isoenzyme PRSI to 0.1 mg/ml lacking Mg2+ and ATP results in loss of 10% activity after 3h at 4ºC | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | 2,3-diphosphoglycerate | at 1 mM, inhibition of isoenzyme PRSI and PRSII, at 1-10 mM, stimulation of isoenzyme PRSI | Homo sapiens | |
2.7.6.1 | ADP | the most effective | Homo sapiens | |
2.7.6.1 | Ca2+ | - |
Homo sapiens | |
2.7.6.1 | CDP | no effect at 1 mM | Homo sapiens | |
2.7.6.1 | CTP | no effect at 1 mM | Homo sapiens | |
2.7.6.1 | GDP | - |
Homo sapiens | |
2.7.6.1 | GMP | no effect at 1 mM | Homo sapiens | |
2.7.6.1 | UDP | no effect at 1 mM | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.021 | - |
ATP | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
2.7.6.1 | 0.021 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
2.7.6.1 | 0.052 | - |
D-ribose 5-phosphate | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
2.7.6.1 | 0.07 | - |
Mg2+ | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
2.7.6.1 | 0.083 | - |
D-ribose 5-phosphate | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens | |
2.7.6.1 | 0.11 | - |
Mg2+ | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens | |
2.7.6.1 | 0.7 | - |
phosphate | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
2.7.6.1 | 2.1 | - |
phosphate | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | Mg2+ | requirement | Homo sapiens | |
2.7.6.1 | Mg2+ | the most effective | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | additional information | - |
- |
Homo sapiens |
2.7.6.1 | 1000000 | - |
gel filtration, isoenzymes PRSI and PRSII | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | Homo sapiens | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.6.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.6.1 | streptomycin precipitation and ammonium sulfate fractionation for isoenzyme PRSI and polyethylene glycol, acid and ammonium sulfate precipitation for isoenzyme PRSII | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 25 | - |
isoenzyme PRSI | Homo sapiens |
2.7.6.1 | 35.7 | - |
isoenzyme PRSII | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.7.6.1 | ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 37 | - |
assay at | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 55 | - |
isoenzyme PRSII is more thermolabile than PRSI. After 10 min, isoenzyme PRSI loses about 30% of activity, isoenzyme PRSII, about 85% of activity | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 7.4 | - |
isoenzyme PRSII | Homo sapiens |
2.7.6.1 | 7.5 | - |
at 40-50 mM phosphate, isoenzymes PRSI and PRSII | Homo sapiens |
2.7.6.1 | 8.8 | - |
isoenzyme PRSI | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 6.5 | 9.3 | At pH 6.5, about 25% and 50% of maximum activity for isoenzymes PRSI and PRSII, respectively. At pH 9.3, about 40% and 60% of maximum activity for isoenzymes PRSII and PRSI, respectively | Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 7.4 | - |
85% of maximal activity, isoenzyme PRSI | Homo sapiens |
2.7.6.1 | 8.8 | - |
41% of maximal activity, isoenzyme PRSII | Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.6.1 | Homo sapiens | two-dimensional protein electrophoresis, isoenzyme PRSI | - |
5.5 |
2.7.6.1 | Homo sapiens | two-dimensional protein electrophoresis, isoenzyme PRSII | - |
5.7 |