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Literature summary extracted from

  • Arnvig, K.; Hove-Jensen, B.; Switzer, R.L.
    Purification and properties of phosphoribosyl-diphosphate synthetase from Bacillus subtilis (1990), Eur. J. Biochem., 192, 195-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate requirement Bacillus subtilis
2.7.6.1 phosphate the optimum concentration at pH 8.2 is 50-60 mM, higher concentrations are inhibitory Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression in Escherichia coli Bacillus subtilis

General Stability

EC Number General Stability Organism
2.7.6.1 relatively stable in absence of phosphate Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP the most effective Bacillus subtilis
2.7.6.1 AMP 68% inhibition at 5 mM Bacillus subtilis
2.7.6.1 ATP moderate inhibition above 3 mM Bacillus subtilis
2.7.6.1 GDP 64% inhibition at 5 mM Bacillus subtilis
2.7.6.1 GMP 88% inhibition at 5 mM Bacillus subtilis
2.7.6.1 L-histidine no inhibition Bacillus subtilis
2.7.6.1 L-tryptophan no inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.48
-
D-ribose 5-phosphate pH 8.2, 37ºC Bacillus subtilis
2.7.6.1 0.66
-
ATP pH 8.2, 37ºC Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ requirement Bacillus subtilis
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Bacillus subtilis
2.7.6.1 Mg2+ the most effective Bacillus subtilis
2.7.6.1 Mn2+ can partially replace Mg2+ Bacillus subtilis
2.7.6.1 Mn2+ 25-30% of the activity with Mg2+ Bacillus subtilis
2.7.6.1 additional information absolute requirement for a divalent cation for activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 34000
-
8 * 34000, SDS-PAGE Bacillus subtilis
2.7.6.1 280000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Bacillus subtilis the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 streptomycin-heat treatment, ammonium sulfate fractionation, chromatography on DEAE-Sepharose and affinity chromatography Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate sequential kinetic mechanism Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 190
-
30fold to 50fold more activity in the cloned enzyme than in the enzyme from the wild-type cells Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -70ºC, glycerol 10%, stable Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Bacillus subtilis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Bacillus subtilis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 octamer 8 * 34000, SDS-PAGE Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8 8.5
-
Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.1 7 9.5 at pH 7 and pH 9.5, about 35% and 85% of the maximal activity, respectively Bacillus subtilis