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Literature summary extracted from

  • Griffith, T.J.; Helleiner, C.W.
    The partial purification of deoxynucleoside monophosphate kinases from L cells (1965), Biochim. Biophys. Acta, 108, 114-124.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.8 additional information no activation by EDTA Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 ATP
-
 Mus musculus
2.7.4.8 EDTA
-
 Mus musculus
2.7.4.8 p-hydroxymercuribenzoate no effect at 0.25 mM, 30% activity at 2.5 mM Mus musculus
2.7.4.11 ATP substrate inhibition Mus musculus
2.7.4.11 EDTA
-
 Mus musculus
2.7.4.11 additional information no inhibition by p-hydroxymercuribenzoate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.072
-
 dGMP pH 8.0, 37ºC Mus musculus
2.7.4.8 0.26
-
 GMP pH 8.0, 37ºC Mus musculus
2.7.4.8 1
-
 ATP pH 8.0, 37ºC, dGMP kinase activity Mus musculus
2.7.4.11 0.1
-
 ATP
-
 Mus musculus
2.7.4.11 1
-
 AMP cosubstrate ATP Mus musculus
2.7.4.11 1
-
 dAMP cosubstrate ATP Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Ca2+ 10% as effective as Mg2+ Mus musculus
2.7.4.8 Co2+ less than 7% as effective as Mg2+ Mus musculus
2.7.4.8 Fe2+ activation, 90% as effective as Mg2+ Mus musculus
2.7.4.8 Fe3+ activation, less than 7% as effective as Mg2+ Mus musculus
2.7.4.8 K+ activation Mus musculus
2.7.4.8 K+ activity of dGMP kinase progressively enhanced as the concentration of KCl is increased to 250 mM, GMP phosphorylation unaffected Mus musculus
2.7.4.8 K+ dGMP, not GMP as substrate Mus musculus
2.7.4.8 Mg2+ requirement Mus musculus
2.7.4.8 Mn2+ requirement Mus musculus
2.7.4.8 Mn2+ equally effective as Mg2+ Mus musculus
2.7.4.8 Zn2+ less than 7% as effective as Mg2+ Mus musculus
2.7.4.11 Co2+ requirement, half as effective as Mg2+ or Mn2+ Mus musculus
2.7.4.11 Fe2+ requirement, half as effective as Mg2+ or Mn2+ Mus musculus
2.7.4.11 Fe3+ activation, only about 15% as effective as Mg2+ or Mn2+ Mus musculus
2.7.4.11 Mg2+ requirement, as good as Mn2+ Mus musculus
2.7.4.11 Mn2+ requirement, as good as Mg2+ Mus musculus
2.7.4.11 additional information no activation by Ca2+, Zn2+ or KCl Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.11 ATP + dAMP Mus musculus
-
 ADP + dADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Mus musculus
-
 mouse
-
2.7.4.11 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 partial by a method that includes DEAE-cellulose chromatography Mus musculus
2.7.4.11 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 fibroblast strain L60TM, a subline of Earle's L-strain, i.e. L-cells Mus musculus
-
2.7.4.11 fibroblast
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 1.24
-
-
 Mus musculus
2.7.4.11 0.6
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + dGMP
-
 Mus musculus ADP + dGDP
-
 r
2.7.4.8 ATP + GMP specificity Mus musculus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Mus musculus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Mus musculus ADP + GDP
-
 ?
2.7.4.11 ATP + AMP
-
 Mus musculus ADP + ADP
-
 ?
2.7.4.11 ATP + dAMP
-
 Mus musculus ADP + dADP
-
 ?
2.7.4.11 ATP + dAMP no substrates are GMP or dGMP Mus musculus ADP + dADP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 37
-
 assay at Mus musculus
2.7.4.11 37
-
 assay at Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.8 80
-
 incubation for 2 min, 5 min, 10 min or 20 min leads to 43%, 67%, 89% or 97% loss of activity, respectively, 30 min: inactivation Mus musculus
2.7.4.11 80
-
 94%, 97%, 98% or 100% loss of activity within 2, 5, 20 or 30 min, respectively Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7 9 equal activity in Tris-HCl buffer and 3,3-dimethylglutarate buffer Mus musculus
2.7.4.8 8
-
 assay at Mus musculus
2.7.4.11 7 9 broad, Tris-HCl preferred to 3,3-dimethylglutarate buffer Mus musculus