Any feedback?
Literature summary extracted from
- Nucleoside diphosphate kinase from brain. Purification and effect on microtubule assembly in vitro (1984), Eur. J. Biochem., 144, 233-241.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.4.6 | cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 17000 | - |
6 * 17000, SDS-PAGE | Sus scrofa |
| 2.7.4.6 | 100000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.4.6 | tubulin-GDP-complex + ATP | Sus scrofa | - |
tubulin-GTP-complex + ADP | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.4.6 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.4.6 | several molecular species can be separated by electrophoresis or chromatofocusing | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.4.6 | brain | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 2026 | - |
25°C | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.4.6 | ATP + tubulin-GDP | - |
Sus scrofa | ADP + tubulin-GTP | - |
? | |
| 2.7.4.6 | GTP + ADP | - |
Sus scrofa | GDP + ATP | - |
r | |
| 2.7.4.6 | NTP + nucleoside diphosphate | - |
Sus scrofa | NDP + NTP | - |
? | |
| 2.7.4.6 | tubulin-GDP-complex + ATP | - |
Sus scrofa | tubulin-GTP-complex + ADP | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.4.6 | hexamer | 6 * 17000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 25 | - |
assay at | Sus scrofa |
| 2.7.4.6 | 37 | - |
assay with tubulin-GDP as substrate | Sus scrofa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 67 | - |
10 min, crude, stable in 0.9 M ammonium sulfate at 22% saturation | Sus scrofa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
