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Literature summary extracted from
- Rapid large-scale purification of pig heart nucleoside diphosphate kinase by affinity chromatography on Cibacron Blue 3G-A Sepharose (1981), Anal. Biochem., 113, 207-211.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 17000 | - |
x * 17000, SDS-PAGE | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.4.6 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.4.6 | affinity chromatography on Blue Sepharose | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.4.6 | heart | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 1500 | - |
25°C, pH 7.4 | Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.4.6 | 4°C, stable overnight | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.4.6 | ATP + 8-bromo-IDP | - |
Sus scrofa | ADP + ? | - |
? |  |
| 2.7.4.6 | NTP + nucleoside diphosphate | - |
Sus scrofa | NDP + NTP | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.4.6 | ? | x * 17000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.4.6 | 25 | - |
assay at | Sus scrofa |
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