EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.3 | cloned and expressed in Escherichia coli | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.3 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
2.7.4.3 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Geobacillus stearothermophilus | |
2.7.4.3 | AMP | - |
Escherichia coli | |
2.7.4.3 | AMP | - |
Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.3 | 0.036 | 0.037 | ATP | ADP, 37°C | Geobacillus stearothermophilus | |
2.7.4.3 | 0.038 | 0.04 | AMP | cosubstrate ATP, 30°C | Escherichia coli | |
2.7.4.3 | 0.048 | 0.051 | ATP | cosubstrate AMP, 30°C | Escherichia coli | |
2.7.4.3 | 0.076 | 0.083 | AMP | 37°C | Geobacillus stearothermophilus | |
2.7.4.3 | 0.09 | 0.092 | ADP3- | ADP, 30°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.3 | Zn2+ | not | Escherichia coli | |
2.7.4.3 | Zn2+ | requirement, tightly bound, 0.8 mol Zn2+ per mol protein, atomic absorption spectrophotometry | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.3 | 24135 | - |
1 * 24135, calculated from nucleotide sequence | Geobacillus stearothermophilus |
2.7.4.3 | 24140 | - |
calculated from nucleotide sequence | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.3 | Escherichia coli | - |
- |
- |
2.7.4.3 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.3 | 2400 | - |
- |
Escherichia coli |
2.7.4.3 | 3000 | - |
- |
Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.3 | ADP + ADP | - |
Escherichia coli | ATP + AMP | - |
r | |
2.7.4.3 | ADP + ADP | - |
Geobacillus stearothermophilus | ATP + AMP | - |
r | |
2.7.4.3 | ATP + AMP | - |
Escherichia coli | ADP + ADP | - |
r | |
2.7.4.3 | ATP + AMP | - |
Geobacillus stearothermophilus | ADP + ADP | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.3 | monomer | - |
Escherichia coli |
2.7.4.3 | monomer | 1 * 24135, calculated from nucleotide sequence | Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.3 | 45 | - |
- |
Escherichia coli |
2.7.4.3 | 65 | - |
- |
Geobacillus stearothermophilus |