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Literature summary extracted from

  • Glaser, P.; Prescecan, E.; Delepierre, M.; Surewicz, W.K.; Mantsch, H.H.; Bârzu, O.; Gilles, A.M.
    Zinc, a novel structural element found in the family of bacterial adenylate kinases (1992), Biochemistry, 31, 3038-3043.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 cloned and expressed in Escherichia coli Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Escherichia coli
2.7.4.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Geobacillus stearothermophilus
2.7.4.3 AMP
-
 Escherichia coli
2.7.4.3 AMP
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.036 0.037 ATP ADP, 37°C Geobacillus stearothermophilus
2.7.4.3 0.038 0.04 AMP cosubstrate ATP, 30°C Escherichia coli
2.7.4.3 0.048 0.051 ATP cosubstrate AMP, 30°C Escherichia coli
2.7.4.3 0.076 0.083 AMP 37°C Geobacillus stearothermophilus
2.7.4.3 0.09 0.092 ADP3- ADP, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Zn2+ not Escherichia coli
2.7.4.3 Zn2+ requirement, tightly bound, 0.8 mol Zn2+ per mol protein, atomic absorption spectrophotometry Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 24135
-
 1 * 24135, calculated from nucleotide sequence Geobacillus stearothermophilus
2.7.4.3 24140
-
 calculated from nucleotide sequence Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Escherichia coli
-
-
-
2.7.4.3 Geobacillus stearothermophilus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 2400
-
-
 Escherichia coli
2.7.4.3 3000
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Escherichia coli ATP + AMP
-
 r
2.7.4.3 ADP + ADP
-
 Geobacillus stearothermophilus ATP + AMP
-
 r
2.7.4.3 ATP + AMP
-
 Escherichia coli ADP + ADP
-
 r
2.7.4.3 ATP + AMP
-
 Geobacillus stearothermophilus ADP + ADP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.3 monomer
-
 Escherichia coli
2.7.4.3 monomer 1 * 24135, calculated from nucleotide sequence Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 45
-
-
 Escherichia coli
2.7.4.3 65
-
-
 Geobacillus stearothermophilus