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Literature summary extracted from

  • McKellar, I.R.C.; Charles, A.M.; Butler, B.J.
    Some properties of adenylate kinase from chemolithotropically grown Thiobacillus novellus (1980), Arch. Microbiol., 124, 275-284.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 3-phosphoglyceraldehyde
-
 Starkeya novella
2.7.4.3 acetyl-CoA
-
 Starkeya novella
2.7.4.3 AMP product inhibition Starkeya novella
2.7.4.3 ATP product inhibition Starkeya novella
2.7.4.3 CoA
-
 Starkeya novella
2.7.4.3 diphosphate reverse reaction Starkeya novella
2.7.4.3 Hg2+ strong, not reaction of ADP + ADP Starkeya novella
2.7.4.3 Mg2+ above 0.5 mM Starkeya novella
2.7.4.3 additional information effect of varios intermediary metabolites; no inhibition by K+, Na+, NH4+, AsO2, citrate, NADH, fructose 6-phosphate, 2-phosphoglyceraldehyde Starkeya novella
2.7.4.3 N-ethylmaleimide not Starkeya novella
2.7.4.3 p-hydroxymercuribenzoate strong, reversible by GSH or cysteine Starkeya novella
2.7.4.3 phosphoenolpyruvate
-
 Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.038 0.04 AMP pH 7.5, 25°C, ADP production Starkeya novella
2.7.4.3 0.09 0.092 ADP3- pH 8.7, 25°C Starkeya novella
2.7.4.3 0.09 0.092 ADP3- ATP production Starkeya novella
2.7.4.3 0.34 0.35 ADP pH 8.7, 25°C Starkeya novella

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Starkeya novella 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Co2+ about 50% as effective as Mg2+ Starkeya novella
2.7.4.3 Mg2+ no absolute requirement: 20% of activity in its absence Starkeya novella
2.7.4.3 Mn2+ requirement, about 25% as effective as Mg2+ Starkeya novella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 40000
-
 sucrose density gradient centrifugation Starkeya novella
2.7.4.3 46300
-
 1 * 46300, SDS-PAGE Starkeya novella
2.7.4.3 47800
-
 1 * 47800, SDS-PAGE after treatment with 5 M urea Starkeya novella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.3 ADP + ADP Starkeya novella involved in energy metabolism ?
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 partial Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 32
-
 pH 8.7, 25°C, ATP production Starkeya novella

Storage Stability

EC Number Storage Stability Organism
2.7.4.3 -20°C, several weeks Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP no substrate: CDP Starkeya novella ATP + AMP
-
 r
2.7.4.3 ADP + ADP no substrates: IDP, GDP Starkeya novella ATP + AMP
-
 r
2.7.4.3 ADP + ADP involved in energy metabolism Starkeya novella ?
-
 r
2.7.4.3 ATP + AMP no substrates are adenosine, 2',3'-AMP or 3',5'-AMP Starkeya novella ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrate of the reverse reaction: CDP Starkeya novella ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/IMP Starkeya novella ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates of the reverse reaction: IDP, GDP Starkeya novella ADP + ADP
-
 r
2.7.4.3 ATP + AMP specific for ATP, AMP and ADP Starkeya novella ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/GMP Starkeya novella ADP + ADP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.3 monomer 1 * 46300, SDS-PAGE Starkeya novella
2.7.4.3 monomer 1 * 47800, SDS-PAGE after treatment with 5 M urea Starkeya novella

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 60
-
 5 min, inactivation, 0.5 mM dithiothreitol protects Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 8.2
-
 AMP + ATP Starkeya novella
2.7.4.3 8.7
-
 ADP + ADP Starkeya novella