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Literature summary extracted from

  • Ito, Y.; Tomasselli, A.G.; Noda, L.H.
    ATP:AMP phosphotransferase from bakers yeast. Purification and properties (1980), Eur. J. Biochem., 105, 85-92.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.3 NaCl 0.5 M Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Saccharomyces cerevisiae
2.7.4.3 Mg2+ at high concentrations; required for enzyme activity at low concentrations Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Ba2+ not Saccharomyces cerevisiae
2.7.4.3 Ca2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
2.7.4.3 Ca2+ in decreasing order of efficiency: Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
2.7.4.3 Ca2+ requirement, as good as Mg2+ Saccharomyces cerevisiae
2.7.4.3 Co2+ requirement Saccharomyces cerevisiae
2.7.4.3 Fe2+ not Saccharomyces cerevisiae
2.7.4.3 Mg2+ requirement Saccharomyces cerevisiae
2.7.4.3 Mg2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
2.7.4.3 Mg2+ in decreasing order of efficiency: Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
2.7.4.3 Mg2+ inhibits at high concentrations Saccharomyces cerevisiae
2.7.4.3 Mn2+ binding of substrates also takes place in the absence of metal ions Saccharomyces cerevisiae
2.7.4.3 Mn2+ Mg2+ and Ca2+, equally efficient, Co2+, Mn2+, Ni2+ Saccharomyces cerevisiae
2.7.4.3 additional information no activation by Sr2+ Saccharomyces cerevisiae
2.7.4.3 Zn2+ not Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 27500
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.7.4.3 4°C, pH 6-9, 2 weeks Saccharomyces cerevisiae
2.7.4.3 deep frozen, pH 6-9, 20 mg enzyme/ml, 4 months, but 60% loss of activity within a few days at pH 5 and 10 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP best substrates Saccharomyces cerevisiae ADP + ADP
-
 ?
2.7.4.3 ATP + AMP no substrates are ATP/IMP Saccharomyces cerevisiae ADP + ADP
-
 ?
2.7.4.3 ATP + AMP no substrates are ATP/UMP Saccharomyces cerevisiae ADP + ADP
-
 ?
2.7.4.3 ATP + AMP no substrates are ATP/GMP Saccharomyces cerevisiae ADP + ADP
-
 ?
2.7.4.3 ATP + CMP
-
 Saccharomyces cerevisiae ADP + ?
-
 ?
2.7.4.3 ATP + dAMP reaction at 11% the rate of AMP Saccharomyces cerevisiae ADP + dADP
-
 ?
2.7.4.3 CTP + AMP reaction at 13% the rate of ATP Saccharomyces cerevisiae ADP + CDP
-
 ?
2.7.4.3 dATP + AMP at the same rate as ATP Saccharomyces cerevisiae dADP + ADP
-
 ?
2.7.4.3 GTP + AMP reaction at 13% the rate of AMP Saccharomyces cerevisiae ADP + GDP
-
 ?
2.7.4.3 ITP + AMP 9% the rate of ATP Saccharomyces cerevisiae IDP + ADP
-
 ?
2.7.4.3 ITP + AMP not ATP/IMP Saccharomyces cerevisiae IDP + ADP
-
 ?
2.7.4.3 UTP + AMP reaction at 11% the rate of AMP Saccharomyces cerevisiae ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 monomer 1 * 27500, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 30
-
 assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 25
-
 20 h stable, pH 6-9 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 7.8
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.3 6 11 about half-maximal activity at pH 6 and 11, ADP + ADP Saccharomyces cerevisiae
2.7.4.3 6 10 about half-maximal activity at pH 6 and 10, AMP + ATP Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.3 Saccharomyces cerevisiae isoelectric focusing
-
 5.7