BRENDA - Enzyme Database

Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

Mikoulinskaia, G.V.; Gubanov, S.I.; Zimin, A.A.; Kolesnikov, I.V.; Feofanov, S.A.; Miroshnikov, A.I.; Protein Expr. Purif. 27, 195-201 (2003)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
29000
-
1 * 29000, SDS-PAGE
Escherichia virus T5
2.7.4.13
29140
-
analytical equilibrium ultracentrifugation
Escherichia virus T5
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.13
Escherichia virus T5
-
from infected Escherichia coli cells; mutant T5amH128a
-
2.7.4.13
no activity in Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.13
1190fold to homogeneity
Escherichia virus T5
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
166.8
-
purified enzyme, substrate dTMP
Escherichia virus T5
2.7.4.13
232
-
purified enzyme, substrate dAMP
Escherichia virus T5
Storage Stability
EC Number
Storage Stability
Organism
2.7.4.13
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months
Escherichia virus T5
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
dAMP is the best acceptor substrate
642544
Escherichia virus T5
ADP + dADP
-
642544
Escherichia virus T5
r
2.7.4.13
ATP + dCMP
phosphorylated at 36% the rate of dAMP
642544
Escherichia virus T5
ADP + dCDP
-
-
-
r
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642544
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642544
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642544
Escherichia virus T5
ADP + dGDP
-
-
-
r
2.7.4.13
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642544
Escherichia virus T5
ADP + dTDP
-
642544
Escherichia virus T5
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.4.13
monomer
1 * 29000, SDS-PAGE
Escherichia virus T5
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.13
37
-
60 min, stable
Escherichia virus T5
2.7.4.13
42
-
10% loss of activity after 30 min
Escherichia virus T5
2.7.4.13
47
-
50% loss of activity after 30 min
Escherichia virus T5
2.7.4.13
52
-
rapid inactivation, t1/2: 5 min
Escherichia virus T5
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
7
7.5
-
Escherichia virus T5
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.13
additional information
-
active over a broad range
Escherichia virus T5
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.4.13
Escherichia virus T5
determined under partially denaturing condiions
-
4.2
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
29000
-
1 * 29000, SDS-PAGE
Escherichia virus T5
2.7.4.13
29140
-
analytical equilibrium ultracentrifugation
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.13
1190fold to homogeneity
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
166.8
-
purified enzyme, substrate dTMP
Escherichia virus T5
2.7.4.13
232
-
purified enzyme, substrate dAMP
Escherichia virus T5
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.4.13
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
dAMP is the best acceptor substrate
642544
Escherichia virus T5
ADP + dADP
-
642544
Escherichia virus T5
r
2.7.4.13
ATP + dCMP
phosphorylated at 36% the rate of dAMP
642544
Escherichia virus T5
ADP + dCDP
-
-
-
r
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642544
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642544
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642544
Escherichia virus T5
ADP + dGDP
-
-
-
r
2.7.4.13
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642544
Escherichia virus T5
ADP + dTDP
-
642544
Escherichia virus T5
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.4.13
monomer
1 * 29000, SDS-PAGE
Escherichia virus T5
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.13
37
-
60 min, stable
Escherichia virus T5
2.7.4.13
42
-
10% loss of activity after 30 min
Escherichia virus T5
2.7.4.13
47
-
50% loss of activity after 30 min
Escherichia virus T5
2.7.4.13
52
-
rapid inactivation, t1/2: 5 min
Escherichia virus T5
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
7
7.5
-
Escherichia virus T5
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.13
additional information
-
active over a broad range
Escherichia virus T5
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.4.13
Escherichia virus T5
determined under partially denaturing condiions
-
4.2