BRENDA - Enzyme Database

The enzymology of virus-infected bacteria. VI. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T5

Bessman, M.J.; Herriott, S.T.; Van Bibber Orr, M.J.; J. Biol. Chem. 240, 439-445 (1965)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.13
dAMP
competitive, dTMP as substrate
Escherichia virus T5
2.7.4.13
dCMP
competitive, dTMP as substrate
Escherichia virus T5
2.7.4.13
dGMP
competitive, dTMP as substrate
Escherichia virus T5
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.13
0.034
-
dCMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.173
-
dTMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.22
-
dAMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.22
-
dGMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.36
-
ATP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
2.8
-
dUMP
pH 7.5, 37°C
Escherichia virus T5
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.13
Co2+
requirement, can replace Mg2+
Escherichia virus T5
2.7.4.13
Fe2+
activation, less effective than Mg2+, Co2+, Mn2+
Escherichia virus T5
2.7.4.13
Mg2+
requirement, can replace Co2+
Escherichia virus T5
2.7.4.13
Mn2+
requirement, can replace Co2+ or Mg2+
Escherichia virus T5
2.7.4.13
additional information
no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+
Escherichia virus T5
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.13
Escherichia virus T5
-
from infected Escherichia coli cells
-
2.7.4.13
no activity in Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.13
500fold
Escherichia virus T5
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
14.7
-
purified enzyme
Escherichia virus T5
Storage Stability
EC Number
Storage Stability
Organism
2.7.4.13
0°C, 25% loss of activity per day in crude cell extract
Escherichia virus T5
2.7.4.13
4°C, several months, ionic strength 0.1 or above
Escherichia virus T5
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + 5-bromo-dUMP
phosphorylated at 45% the rate of dAMP
642542
Escherichia virus T5
ADP + 5-bromo-dUDP
-
-
-
r
2.7.4.13
ATP + dAMP
dAMP is the best acceptor substrate
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dAMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dCMP
phosphorylated at 30% the rate of dAMP, dATP can replace ATP
642542
Escherichia virus T5
ADP + dCDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642542
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
dATP can replace ATP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dTMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
2.7.4.13
additional information
very poor acceptor substrates are AMP, CMP or dUMP
642542
Escherichia virus T5
?
-
-
-
-
2.7.4.13
additional information
no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine
642542
Escherichia virus T5
?
-
-
-
-
2.7.4.13
additional information
no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP
642542
Escherichia virus T5
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.13
37
-
assay at
Escherichia virus T5
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
7
-
-
Escherichia virus T5
2.7.4.13
7.6
-
substrate dTMP
Escherichia virus T5
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.13
6.2
9
about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP
Escherichia virus T5
2.7.4.13
6.2
9.4
about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP
Escherichia virus T5
2.7.4.13
6.3
9
at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP
Escherichia virus T5
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.4.13
0.032
-
dCMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
2.7.4.13
0.15
-
dAMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
2.7.4.13
0.15
-
dGMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.13
dAMP
competitive, dTMP as substrate
Escherichia virus T5
2.7.4.13
dCMP
competitive, dTMP as substrate
Escherichia virus T5
2.7.4.13
dGMP
competitive, dTMP as substrate
Escherichia virus T5
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.4.13
0.032
-
dCMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
2.7.4.13
0.15
-
dAMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
2.7.4.13
0.15
-
dGMP
dTMP as substrate, pH 7.6, 37°C
Escherichia virus T5
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.13
0.034
-
dCMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.173
-
dTMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.22
-
dAMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.22
-
dGMP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
0.36
-
ATP
pH 7.5, 37°C
Escherichia virus T5
2.7.4.13
2.8
-
dUMP
pH 7.5, 37°C
Escherichia virus T5
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.13
Co2+
requirement, can replace Mg2+
Escherichia virus T5
2.7.4.13
Fe2+
activation, less effective than Mg2+, Co2+, Mn2+
Escherichia virus T5
2.7.4.13
Mg2+
requirement, can replace Co2+
Escherichia virus T5
2.7.4.13
Mn2+
requirement, can replace Co2+ or Mg2+
Escherichia virus T5
2.7.4.13
additional information
no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.13
500fold
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
14.7
-
purified enzyme
Escherichia virus T5
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.4.13
0°C, 25% loss of activity per day in crude cell extract
Escherichia virus T5
2.7.4.13
4°C, several months, ionic strength 0.1 or above
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + 5-bromo-dUMP
phosphorylated at 45% the rate of dAMP
642542
Escherichia virus T5
ADP + 5-bromo-dUDP
-
-
-
r
2.7.4.13
ATP + dAMP
dAMP is the best acceptor substrate
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dAMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dADP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dCMP
phosphorylated at 30% the rate of dAMP, dATP can replace ATP
642542
Escherichia virus T5
ADP + dCDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642542
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
dATP can replace ATP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642542
Escherichia virus T5
ADP + dGDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dTMP
dATP can replace ATP with less efficiency
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
2.7.4.13
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642542
Escherichia virus T5
ADP + dTDP
-
642542
Escherichia virus T5
r
2.7.4.13
additional information
very poor acceptor substrates are AMP, CMP or dUMP
642542
Escherichia virus T5
?
-
-
-
-
2.7.4.13
additional information
no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine
642542
Escherichia virus T5
?
-
-
-
-
2.7.4.13
additional information
no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP
642542
Escherichia virus T5
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.13
37
-
assay at
Escherichia virus T5
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
7
-
-
Escherichia virus T5
2.7.4.13
7.6
-
substrate dTMP
Escherichia virus T5
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.13
6.2
9
about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP
Escherichia virus T5
2.7.4.13
6.2
9.4
about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP
Escherichia virus T5
2.7.4.13
6.3
9
at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP
Escherichia virus T5