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Literature summary extracted from

  • Haas, D.; Leisinger, T.
    N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Catalytic and regulatory properties (1975), Eur. J. Biochem., 52, 377-383.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 ADP
-
 Pseudomonas aeruginosa
2.7.2.8 arginine feedback inhibition is markedly dependent on pH, above pH 9 no inhibition Pseudomonas aeruginosa
2.7.2.8 L-citrulline
-
 Pseudomonas aeruginosa
2.7.2.8 MgCl2 30 mM, slight inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 2
-
 N-acetyl-L-glutamate pH 7.2, 37°C Pseudomonas aeruginosa
2.7.2.8 3
-
 ATP pH 7.2, 37°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.8 Ca2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa
2.7.2.8 Co2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa
2.7.2.8 Mg2+ required Pseudomonas aeruginosa
2.7.2.8 Mg2+ 30 mM, slight inhibition Pseudomonas aeruginosa
2.7.2.8 Mn2+ Mn2+, Zn2+, Co2+ and Ca2+ in decreasing order can partially replace Mg2+ Pseudomonas aeruginosa
2.7.2.8 Zn2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
2.7.2.8 ATP + N-carbamoyl-L-glutamate at 33% of the activity with N-acetyl-L-glutamate Pseudomonas aeruginosa ADP + N-carbamoyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-formyl-L-glutamate at 20% of the activity with N-acetyl-L-glutamate Pseudomonas aeruginosa ADP + N-formyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 dATP + N-acetyl-L-glutamate as effective as ATP Pseudomonas aeruginosa dADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 additional information not: ITP Pseudomonas aeruginosa ?
-
 ?
2.7.2.8 additional information not: GTP Pseudomonas aeruginosa ?
-
 ?
2.7.2.8 additional information N-propionyl-L-glutamate almost inactive Pseudomonas aeruginosa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.8 6.5 9
-
 Pseudomonas aeruginosa