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Literature summary extracted from

  • Denes, G.
    N-Acetylglutamate-5-phosphotransferase (1973), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 9, 511-520.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.2.8 L-arginine protects against the inactivating effects of heat Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 arginine allosteric enzyme which is inhibited by arginine Chlamydomonas reinhardtii
2.7.2.8 arginine enzyme has no allosteric properties and its activity is influenced neither by arginine nor by any of the intermediates of the arginine biosynthetic pathway Escherichia coli
2.7.2.8 MgCl2 inhibition above 20 mM Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 1
-
 ATP
-
 Escherichia coli
2.7.2.8 3
-
 ATP pH 8.0, 37°C, allosteric Chlamydomonas reinhardtii
2.7.2.8 4
-
 N-acetyl-L-glutamate pH 8.0, 37°C, allosteric Chlamydomonas reinhardtii
2.7.2.8 6
-
 N-acetyl-L-glutamate pH 5.5, 37°C Escherichia coli
2.7.2.8 15
-
 N-acetyl-L-glutamate
-
 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.8 Co2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii
2.7.2.8 Mg2+
-
 Escherichia coli
2.7.2.8 Mg2+ required Chlamydomonas reinhardtii
2.7.2.8 Mg2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 400000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate Chlamydomonas reinhardtii key enzyme in regulation of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate Escherichia coli key enzyme in regulation of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Chlamydomonas reinhardtii
-
-
-
2.7.2.8 Escherichia coli
-
 ATCC 25542
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8
-
 Chlamydomonas reinhardtii
2.7.2.8
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate highly specific for ATP and N-acetyl-L-glutamate Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
2.7.2.8 ATP + N-acetyl-L-glutamate highly specific for ATP and N-acetyl-L-glutamate Escherichia coli ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
2.7.2.8 ATP + N-acetyl-L-glutamate key enzyme in regulation of arginine biosynthesis Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate key enzyme in regulation of arginine biosynthesis Escherichia coli ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 additional information not: GTP Chlamydomonas reinhardtii ?
-
 ?
2.7.2.8 additional information not: GTP Escherichia coli ?
-
 ?
2.7.2.8 additional information not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate Chlamydomonas reinhardtii ?
-
 ?
2.7.2.8 additional information not: N-benzoyl-L-glutamate, L-glutamate, D-glutamate Escherichia coli ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.8 additional information
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.8 5.5
-
-
 Chlamydomonas reinhardtii
2.7.2.8 6.8 7.8
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.8 5.5 8.5
-
 Chlamydomonas reinhardtii