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Literature summary extracted from

  • Hartmanis, M.G.N.
    Butyrate kinase from Clostridium acetobutylicum (1987), J. Biol. Chem., 262, 617-621.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.7 DTT presence of a reducing agent is required Clostridium acetobutylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.7 iodoacetamide 20 mM; 49% inhibition Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.7 14
-
 Butanoate pH 7.6 Clostridium acetobutylicum
2.7.2.7 14
-
 valerate pH 7.6 Clostridium acetobutylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.7 Mg2+ Km: 2.0 mM Clostridium acetobutylicum
2.7.2.7 Mn2+ Km: 4.0 mM Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.7 39000
-
 2 * 39000, SDS-PAGE Clostridium acetobutylicum
2.7.2.7 85000
-
 non-denaturing PAGE Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.7 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.7
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.7 402
-
-
 Clostridium acetobutylicum

Storage Stability

EC Number Storage Stability Organism
2.7.2.7 -20°C, 25 mM Tris-HCl, pH 7.6, 1 mM DTT, stable for several months Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.7 ATP + acetate 6% of the activity with butanoate Clostridium acetobutylicum ADP + acetyl phosphate
-
 ?
2.7.2.7 ATP + butanoate
-
 Clostridium acetobutylicum ADP + butanoyl phosphate
-
 r
2.7.2.7 ATP + isobutanoate 54% of the activity with butanoate Clostridium acetobutylicum ADP + isobutanoyl phosphate
-
 ?
2.7.2.7 ATP + isovalerate 32% of the activity with butanoate Clostridium acetobutylicum ADP + isopentanoyl phosphate
-
 ?
2.7.2.7 ATP + propionate 43% of the activity with butanoate Clostridium acetobutylicum ADP + propanoyl phosphate
-
 ?
2.7.2.7 ATP + valerate 89% of the activity with butanoate Clostridium acetobutylicum ADP + pentanoyl phosphate
-
 ?
2.7.2.7 ATP + vinyl acetate 23% of the activity with butanoate Clostridium acetobutylicum ADP + vinylacetyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.7 dimer 2 * 39000, SDS-PAGE Clostridium acetobutylicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.7 50
-
 20 min, 70% loss of activity in absence of 100 mM butanoate, complete protection against inactivation in presence of mM potassium butanoate Clostridium acetobutylicum
2.7.2.7 60
-
 1 min, complete loss of activity in absence of butanoate Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.7 7.5
-
 formation of butanoyl phosphate Clostridium acetobutylicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.7 6 10 pH 6: about 25% of maximal activity, pH 10.0: about 50% of maximal activity Clostridium acetobutylicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.7 6.5 9.5 very stable Clostridium acetobutylicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.7 Clostridium acetobutylicum isoelectric focusing 5.9 5.6