Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kobashi, N.; Nishiyama, M.; Tanokura, M.
    Kinetic and mutation analyses of aspartate kinase from Thermus flavus (1999), J. Biosci. Bioeng., 87, 739-745.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.4 L-threonine 0.01 mM, mutant G135A, activity 110%, mutant R150A, activity 115% Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 2 subunits, alpha and beta, encoded by an inframe overlapping gene, askAB genes cloned and expressed in Escherichia coli GT3 Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.4 A157L site-directed mutagenesis Thermus thermophilus
2.7.2.4 A189L site-directed mutagenesis Thermus thermophilus
2.7.2.4 A23L site-directed mutagenesis Thermus thermophilus
2.7.2.4 A42S site-directed mutagenesis Thermus thermophilus
2.7.2.4 D154A site-directed mutagenesis Thermus thermophilus
2.7.2.4 D154N site-directed mutagenesis Thermus thermophilus
2.7.2.4 D174A site-directed mutagenesis Thermus thermophilus
2.7.2.4 D182A site-directed mutagenesis Thermus thermophilus
2.7.2.4 E202A site-directed mutagenesis Thermus thermophilus
2.7.2.4 F136A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G10A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G135A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G149A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G152A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G177A site-directed mutagenesis Thermus thermophilus
2.7.2.4 G73A site-directed mutagenesis Thermus thermophilus
2.7.2.4 I171A site-directed mutagenesis Thermus thermophilus
2.7.2.4 L148A site-directed mutagenesis Thermus thermophilus
2.7.2.4 P183A site-directed mutagenesis Thermus thermophilus
2.7.2.4 R150A site-directed mutagenesis Thermus thermophilus
2.7.2.4 S12A site-directed mutagenesis Thermus thermophilus
2.7.2.4 S153A site-directed mutagenesis Thermus thermophilus
2.7.2.4 S41A site-directed mutagenesis Thermus thermophilus
2.7.2.4 T238A site-directed mutagenesis Thermus thermophilus
2.7.2.4 T47A site-directed mutagenesis Thermus thermophilus
2.7.2.4 V39A site-directed mutagenesis Thermus thermophilus
2.7.2.4 Y8A site-directed mutagenesis Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 0.19
-
 L-aspartate pH 7.5, 60°C, mutant A157L Thermus thermophilus
2.7.2.4 0.27
-
 L-aspartate pH 7.5, 60°C, mutant E202A Thermus thermophilus
2.7.2.4 0.27
-
 ATP pH 7.5, 60°C, mutant G177A Thermus thermophilus
2.7.2.4 0.29
-
 ATP pH 7.5, 60°C, mutant G149A Thermus thermophilus
2.7.2.4 0.32
-
 L-aspartate pH 7.5, 60°C, mutant Y8A, S12A Thermus thermophilus
2.7.2.4 0.33
-
 L-aspartate pH 7.5, 60°C, mutant V39A Thermus thermophilus
2.7.2.4 0.34
-
 L-aspartate pH 7.5, 60°C, mutant G177A Thermus thermophilus
2.7.2.4 0.36
-
 L-aspartate pH 7.5, 60°C, mutant G149A, G177A Thermus thermophilus
2.7.2.4 0.37
-
 L-aspartate pH 7.5, 60°C, mutant I171A Thermus thermophilus
2.7.2.4 0.39
-
 L-aspartate pH 7.5, 60°C, mutant T238A Thermus thermophilus
2.7.2.4 0.41
-
 ATP pH 7.5, 60°C, mutant G149A Thermus thermophilus
2.7.2.4 0.41
-
 L-aspartate pH 7.5, 60°C, mutant S41A Thermus thermophilus
2.7.2.4 0.42
-
 L-aspartate pH 7.5, 60°C, mutant G152A, P183A Thermus thermophilus
2.7.2.4 0.43
-
 ATP pH 7.5, 60°C, mutant D174A Thermus thermophilus
2.7.2.4 0.44
-
 ATP pH 7.5, 60°C, mutant S153A Thermus thermophilus
2.7.2.4 0.45
-
 ATP pH 7.5, 60°C, mutant A157L Thermus thermophilus
2.7.2.4 0.45
-
 L-aspartate pH 7.5, 60°C, mutant A189L Thermus thermophilus
2.7.2.4 0.46
-
 ATP pH 7.5, 60°C, mutant G150A Thermus thermophilus
2.7.2.4 0.47
-
 L-aspartate pH 7.5, 60°C, mutant S153A Thermus thermophilus
2.7.2.4 0.5
-
 ATP pH 7.5, 60°C, wild-type and mutant G152A Thermus thermophilus
2.7.2.4 0.52
-
 ATP pH 7.5, 60°C, mutant S12A, G135A Thermus thermophilus
2.7.2.4 0.53
-
 L-aspartate pH 7.5, 60°C, mutant D174A Thermus thermophilus
2.7.2.4 0.56
-
 ATP pH 7.5, 60°C, mutant T238A Thermus thermophilus
2.7.2.4 0.57
-
 ATP pH 7.5, 60°C, mutant F136A Thermus thermophilus
2.7.2.4 0.6
-
 ATP pH 7.5, 60°C, mutant I171A Thermus thermophilus
2.7.2.4 0.6
-
 L-aspartate pH 7.5, 60°C, mutant A42S Thermus thermophilus
2.7.2.4 0.61
-
 L-aspartate pH 7.5, 60°C, mutant G73A Thermus thermophilus
2.7.2.4 0.63
-
 ATP pH 7.5, 60°C, mutant D154N Thermus thermophilus
2.7.2.4 0.65
-
 L-aspartate pH 7.5, 60°C, mutant A23L Thermus thermophilus
2.7.2.4 0.72
-
 ATP pH 7.5, 60°C, mutant A23L Thermus thermophilus
2.7.2.4 0.86
-
 ATP pH 7.5, 60°C, mutant Y8A Thermus thermophilus
2.7.2.4 0.89
-
 ATP pH 7.5, 60°C, mutant V39A Thermus thermophilus
2.7.2.4 0.97
-
 ATP pH 7.5, 60°C, mutant L148A Thermus thermophilus
2.7.2.4 0.98
-
 ATP pH 7.5, 60°C, mutant A189L Thermus thermophilus
2.7.2.4 1.02
-
 ATP pH 7.5, 60°C, mutant A42S Thermus thermophilus
2.7.2.4 1.05
-
 ATP pH 7.5, 60°C, mutant D154A Thermus thermophilus
2.7.2.4 1.2
-
 L-aspartate pH 7.5, 60°C, mutant P183A Thermus thermophilus
2.7.2.4 1.23
-
 L-aspartate pH 7.5, 60°C, mutant R150A, D154A Thermus thermophilus
2.7.2.4 1.28
-
 ATP pH 7.5, 60°C, mutant G73A Thermus thermophilus
2.7.2.4 1.31
-
 L-aspartate pH 7.5, 60°C, D182A Thermus thermophilus
2.7.2.4 1.34
-
 L-aspartate pH 7.5, 60°C, mutant G10A, G135A Thermus thermophilus
2.7.2.4 1.39
-
 ATP pH 7.5, 60°C, mutant S41A Thermus thermophilus
2.7.2.4 1.9
-
 ATP pH 7.5, 60°C, mutant P183A Thermus thermophilus
2.7.2.4 2.1
-
 L-aspartate pH 7.5, 60°C, mutant L148A Thermus thermophilus
2.7.2.4 2.1
-
 ATP pH 7.5, 60°C, mutant T47A Thermus thermophilus
2.7.2.4 2.45
-
 ATP pH 7.5, 60°C, mutant D182A Thermus thermophilus
2.7.2.4 3.57
-
 ATP pH 7.5, 60°C, mutant G10A Thermus thermophilus
2.7.2.4 5.29
-
 L-aspartate pH 7.5, 60°C, mutant F136A Thermus thermophilus
2.7.2.4 19.5
-
 L-aspartate pH 7.5, 60°C, mutant T47A Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 Mg2+ required for activity Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 17000
-
 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus
2.7.2.4 43000
-
 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Thermus thermophilus first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine ADP + 4-phospho-L-aspartate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 recombinant enzyme, expressed in E. coli Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 12
-
-
 Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
2.7.2.4 4°C, purified enzyme stable for at least 2 weeks in gel filtration buffer Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Thermus thermophilus ADP + 4-phospho-L-aspartate
-
 r
2.7.2.4 ATP + L-aspartate first step of the branched biosynthetic pathway for lysine, threonine, isoleucine and methionine Thermus thermophilus ADP + 4-phospho-L-aspartate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.4 heterodimer 1 * 17000 + 1 * 43000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.4 0.8
-
 L-aspartate pH 7.5, 60°C, mutant S41A Thermus thermophilus
2.7.2.4 0.967
-
 L-aspartate pH 7.5, 60°C, mutant G135A Thermus thermophilus
2.7.2.4 1.83
-
 L-aspartate pH 7.5, 60°C, mutant R150A, D154A Thermus thermophilus
2.7.2.4 2.17
-
 L-aspartate pH 7.5, 60°C, mutant D154N, D182A Thermus thermophilus
2.7.2.4 2.83
-
 L-aspartate pH 7.5, 60°C, mutant G10A Thermus thermophilus
2.7.2.4 3
-
 L-aspartate pH 7.5, 60°C, mutant D174A Thermus thermophilus
2.7.2.4 4
-
 L-aspartate pH 7.5, 60°C, mutant E202A Thermus thermophilus
2.7.2.4 4.33
-
 L-aspartate pH 7.5, 60°C, mutant G152A, P183A Thermus thermophilus
2.7.2.4 4.67
-
 L-aspartate pH 7.5, 60°C, mutant A157L Thermus thermophilus
2.7.2.4 6
-
 L-aspartate pH 7.5, 60°C, mutant A189L Thermus thermophilus
2.7.2.4 6.67
-
 L-aspartate pH 7.5, 60°C, mutant T47A Thermus thermophilus
2.7.2.4 7.33
-
 L-aspartate pH 7.5, 60°C, mutant T238A Thermus thermophilus
2.7.2.4 10.8
-
 L-aspartate pH 7.5, 60°C, mutant I171A Thermus thermophilus
2.7.2.4 12.3
-
 L-aspartate pH 7.5, 60°C, mutant S153A Thermus thermophilus
2.7.2.4 13.3
-
 L-aspartate pH 7.5, 60°C, mutant G149A, G177A Thermus thermophilus
2.7.2.4 13.8
-
 L-aspartate pH 7.5, 60°C, mutant Y8A Thermus thermophilus
2.7.2.4 16.7
-
 L-aspartate pH 7.5, 60°C, mutant F136A Thermus thermophilus
2.7.2.4 23.2
-
 L-aspartate pH 7.5, 60°C, mutant A42S Thermus thermophilus
2.7.2.4 23.3
-
 L-aspartate pH 7.5, 60°C, mutant L148A Thermus thermophilus
2.7.2.4 24.3
-
 L-aspartate pH 7.5, 60°C, mutant A23L Thermus thermophilus
2.7.2.4 24.7
-
 L-aspartate pH 7.5, 60°C, mutant S12A Thermus thermophilus
2.7.2.4 25
-
 L-aspartate pH 7.5, 60°C, wild-type Thermus thermophilus
2.7.2.4 31.7
-
 L-aspartate pH 7.5, 60°C, mutant G73A Thermus thermophilus
2.7.2.4 32
-
 L-aspartate pH 7.5, 60°C, mutant V39A Thermus thermophilus