BRENDA - Enzyme Database

Specificity of aspartokinase III from Escherichia coli and an examination of important catalytic residues

Keng, Y.F.; Viola, R.E.; Arch. Biochem. Biophys. 335, 73-81 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.2.4
lysC geneencoding for AKIII cloned into vector pTZ19U and expressed in the protease-deficient Escherichia coli BL21DE3
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.2.4
D-aspartate beta-hydroxamate
-
Escherichia coli
2.7.2.4
D-aspartic acid 1-benzyl ester
-
Escherichia coli
2.7.2.4
Diethyl aminomalonate
-
Escherichia coli
2.7.2.4
diethyldicarbonate
-
Escherichia coli
2.7.2.4
DL-3-amino-4-hydroxybutyrate methyl ester
-
Escherichia coli
2.7.2.4
DL-3-aminobutyrate
-
Escherichia coli
2.7.2.4
L-2-aminobutyrate
competitive inhibitor
Escherichia coli
2.7.2.4
L-glutamate
-
Escherichia coli
2.7.2.4
L-glutamate gamma-methyl ester
-
Escherichia coli
2.7.2.4
L-homoserine
-
Escherichia coli
2.7.2.4
L-malate
-
Escherichia coli
2.7.2.4
L-norvaline
-
Escherichia coli
2.7.2.4
malonate
-
Escherichia coli
2.7.2.4
N-Acetylimidazole
-
Escherichia coli
2.7.2.4
N-ethylmaleimide
-
Escherichia coli
2.7.2.4
succinate
-
Escherichia coli
2.7.2.4
Tetranitromethane
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.6
-
L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
2.5
-
L-aspartate beta-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
4.9
-
L-aspartate beta-methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
5.3
-
L-aspartic acid 1-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
13
-
L-aspartate beta-hydroxamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
16
-
L-asparagine
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
41
-
N-formyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
48
-
N-acetyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
68
-
N-Chloroacetyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
147
-
DL-threo-3-methyl aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
184
-
L-aspartic acid amide
pH 8.0, 25°C, aspartokinase III
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.2.4
50000
-
2 * 50000
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.2.4
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.2.4
aspartokinase III
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.2.4
ATP + DL-threo-3-methyl aspartate
aspartokinase III
642329
Escherichia coli
ADP + 3-methyl-4-phosphoaspartate
-
-
-
?
2.7.2.4
ATP + L-asparagine
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartate beta-hydroxamate
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartate beta-methyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid 1-benzyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid 4-benzyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid amide
aspartokinase III
642329
Escherichia coli
ADP + 4-phospho-L-aspartic acid amide
-
-
-
?
2.7.2.4
ATP + N-acetyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-acetyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + N-chloroacetyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-chloroacetyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + N-formyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-formyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
additional information
aspartokinase III, D-isomers of the derivatives of aspartic acid, including D-aspartate alpha-benzyl ester and D-aspartate beta-hydroxamate are not substrates regardless of whether the alpha- or the beta-carboxyl group is derivatized, L-cysteine sulfinate and 2-methyl-DL-aspartate are no substrates
642329
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.2.4
dimer
2 * 50000
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.2.4
7
9
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.2.4
6
11
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.2.4
7.7
-
L-homoserine
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
14
-
D-aspartic acid 1-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
14
-
DL-3-amino-4-hydroxybutyrate methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
28
-
L-2-aminobutyrate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
32
-
L-norvaline
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
53
-
L-malate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
63
-
Diethyl aminomalonate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
82
-
L-glutamate gamma-methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
87
-
D-aspartate beta-hydroxamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
108
-
malonate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
128
-
L-glutamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
159
-
succinate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
245
-
DL-3-aminobutyrate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.2.4
lysC geneencoding for AKIII cloned into vector pTZ19U and expressed in the protease-deficient Escherichia coli BL21DE3
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.2.4
D-aspartate beta-hydroxamate
-
Escherichia coli
2.7.2.4
D-aspartic acid 1-benzyl ester
-
Escherichia coli
2.7.2.4
Diethyl aminomalonate
-
Escherichia coli
2.7.2.4
diethyldicarbonate
-
Escherichia coli
2.7.2.4
DL-3-amino-4-hydroxybutyrate methyl ester
-
Escherichia coli
2.7.2.4
DL-3-aminobutyrate
-
Escherichia coli
2.7.2.4
L-2-aminobutyrate
competitive inhibitor
Escherichia coli
2.7.2.4
L-glutamate
-
Escherichia coli
2.7.2.4
L-glutamate gamma-methyl ester
-
Escherichia coli
2.7.2.4
L-homoserine
-
Escherichia coli
2.7.2.4
L-malate
-
Escherichia coli
2.7.2.4
L-norvaline
-
Escherichia coli
2.7.2.4
malonate
-
Escherichia coli
2.7.2.4
N-Acetylimidazole
-
Escherichia coli
2.7.2.4
N-ethylmaleimide
-
Escherichia coli
2.7.2.4
succinate
-
Escherichia coli
2.7.2.4
Tetranitromethane
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.2.4
7.7
-
L-homoserine
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
14
-
D-aspartic acid 1-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
14
-
DL-3-amino-4-hydroxybutyrate methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
28
-
L-2-aminobutyrate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
32
-
L-norvaline
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
53
-
L-malate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
63
-
Diethyl aminomalonate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
82
-
L-glutamate gamma-methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
87
-
D-aspartate beta-hydroxamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
108
-
malonate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
128
-
L-glutamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
159
-
succinate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
245
-
DL-3-aminobutyrate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.6
-
L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
2.5
-
L-aspartate beta-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
4.9
-
L-aspartate beta-methyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
5.3
-
L-aspartic acid 1-benzyl ester
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
13
-
L-aspartate beta-hydroxamate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
16
-
L-asparagine
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
41
-
N-formyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
48
-
N-acetyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
68
-
N-Chloroacetyl-L-aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
147
-
DL-threo-3-methyl aspartate
pH 8.0, 25°C, aspartokinase III
Escherichia coli
2.7.2.4
184
-
L-aspartic acid amide
pH 8.0, 25°C, aspartokinase III
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.2.4
50000
-
2 * 50000
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.2.4
aspartokinase III
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.2.4
ATP + DL-threo-3-methyl aspartate
aspartokinase III
642329
Escherichia coli
ADP + 3-methyl-4-phosphoaspartate
-
-
-
?
2.7.2.4
ATP + L-asparagine
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartate beta-hydroxamate
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartate beta-methyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid 1-benzyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid 4-benzyl ester
aspartokinase III
642329
Escherichia coli
?
-
-
-
?
2.7.2.4
ATP + L-aspartic acid amide
aspartokinase III
642329
Escherichia coli
ADP + 4-phospho-L-aspartic acid amide
-
-
-
?
2.7.2.4
ATP + N-acetyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-acetyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + N-chloroacetyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-chloroacetyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + N-formyl-L-aspartate
aspartokinase III
642329
Escherichia coli
ADP + N-formyl-4-phospho-L-aspartate
-
-
-
?
2.7.2.4
additional information
aspartokinase III, D-isomers of the derivatives of aspartic acid, including D-aspartate alpha-benzyl ester and D-aspartate beta-hydroxamate are not substrates regardless of whether the alpha- or the beta-carboxyl group is derivatized, L-cysteine sulfinate and 2-methyl-DL-aspartate are no substrates
642329
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.2.4
dimer
2 * 50000
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.2.4
7
9
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.2.4
6
11
-
Escherichia coli