Literature summary extracted from

Kochhar, S.; Kochhar, V.K.; Sane, P.V.
Isolation, characterization and regulation of isoenzymes of aspartate kinase differentially sensitive to calmodulin from spinach leaves (1986), Biochim. Biophys. Acta, 880, 220-225.

No PubMed abstract available

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.2.4	Calmodulin	isoenzyme II	Spinacia oleracea

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.2.4	EGTA	isoenzyme II	Spinacia oleracea
2.7.2.4	L-lysine	isoenzyme I	Spinacia oleracea
2.7.2.4	L-threonine	isoenzyme II	Spinacia oleracea

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.2.4	1	-	ATP	pH 7.5, isoenzyme I	Spinacia oleracea
2.7.2.4	1.67	-	ATP	pH 7.5, isoenzyme II	Spinacia oleracea
2.7.2.4	2	-	L-aspartate	pH 7.5, isoenzyme I and II	Spinacia oleracea

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.2.4	Ca2+	isoenzyme I is insensitive to Ca2+ and calmodulin, isoenzyme II is activated by calmodulin, the effect is further increased by Ca2+	Spinacia oleracea

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.2.4	200000	-	isoenzyme II, gel filtration	Spinacia oleracea
2.7.2.4	330000	-	isoenzyme I, gel filtration	Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.7.2.4	ATP + L-aspartate	Spinacia oleracea	-	ADP + 4-phospho-L-aspartate	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.2.4	Spinacia oleracea	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.2.4	leaf	-	Spinacia oleracea	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.2.4	ATP + L-aspartate	-	Spinacia oleracea	ADP + 4-phospho-L-aspartate	-	r

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Release 2023.1 (January 2023)