BRENDA - Enzyme Database show

Microbial aspartokinases

Truffa-Bachi, P.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 8, 509-553 (1973)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.2.4
NH4+
-
Geobacillus stearothermophilus
2.7.2.4
NH4+
-
Paenibacillus polymyxa
General Stability
EC Number
General Stability
Organism
2.7.2.4
L-lysine, L-threonine or L-methionine protects the enzymic activity against heat inactivation
Rhodocyclus tenuis
2.7.2.4
nonpolar L-amino acids protect from inactivation by heat and detergent and reverse the inhibition caused by feedback inhibitors L-lysine and L-threonine
Paenibacillus polymyxa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.2.4
aspartate-beta-semialdehyde
-
Bacillus licheniformis
2.7.2.4
aspartate-beta-semialdehyde
-
Rhodobacter sphaeroides
2.7.2.4
DL-meso-diaminopimelic acid
aspartokinase I, noncompetitive inhibition
Bacillus subtilis
2.7.2.4
DL-meso-diaminopimelic acid
-
Geobacillus stearothermophilus
2.7.2.4
L-lysine
-
Azotobacter sp.
2.7.2.4
L-lysine
-
Bacillus cereus
2.7.2.4
L-lysine
-
Bacillus licheniformis
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Brevibacterium flavum
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Corynebacterium glutamicum
2.7.2.4
L-lysine
-
Geobacillus stearothermophilus
2.7.2.4
L-lysine
-
Paenibacillus polymyxa
2.7.2.4
L-lysine
-
Pseudomonas aeruginosa
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Pseudomonas fluorescens
2.7.2.4
L-lysine
-
Pseudomonas putida
2.7.2.4
L-lysine
-
Rhodobacter capsulatus
2.7.2.4
L-lysine
-
Rhodobacter sphaeroides
2.7.2.4
L-lysine
-
Rhodocyclus tenuis
2.7.2.4
L-lysine
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.4
L-lysine
-
Bacillus subtilis
2.7.2.4
L-lysine
-
Escherichia coli
2.7.2.4
L-threonine
aspartokinase II, competitive inhibition
Bacillus subtilis
2.7.2.4
L-threonine
-
Geobacillus stearothermophilus
2.7.2.4
L-threonine
-
Paenibacillus polymyxa
2.7.2.4
L-threonine
-
Pseudomonas aeruginosa
2.7.2.4
L-threonine
-
Pseudomonas fluorescens
2.7.2.4
L-threonine
-
Pseudomonas putida
2.7.2.4
L-threonine
-
Rhodocyclus tenuis
2.7.2.4
L-threonine
-
Saccharomyces cerevisiae
2.7.2.4
L-threonine
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.18
-
ATP
aspartokinase I
Escherichia coli
2.7.2.4
0.9
-
L-aspartate
-
Rhodocyclus tenuis
2.7.2.4
1.5
-
L-aspartate
aspartokinase I
Escherichia coli
2.7.2.4
1.5
-
L-aspartate
-
Paenibacillus polymyxa
2.7.2.4
1.9
-
ATP
aspartokinase II, 27C
Escherichia coli
2.7.2.4
2.1
-
L-aspartate
aspartokinase II, 27C
Escherichia coli
2.7.2.4
3
-
ATP
-
Rhodocyclus tenuis
2.7.2.4
3
-
L-aspartate
aspartokinase I
Bacillus subtilis
2.7.2.4
4
-
ATP
aspartokinase I
Escherichia coli
2.7.2.4
4.7
-
L-aspartate
aspartokinase III, 27C
Escherichia coli
2.7.2.4
4.8
-
ATP
aspartokinase III, 27C
Escherichia coli
2.7.2.4
4.8
-
ATP
-
Pseudomonas putida
2.7.2.4
4.8
-
L-aspartate
-
Pseudomonas putida
2.7.2.4
17
-
L-aspartate
aspartokinase II
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.2.4
Fe2+
-
Neurospora crassa
2.7.2.4
Fe2+
-
Paenibacillus polymyxa
2.7.2.4
Fe2+
-
Saccharomyces cerevisiae
2.7.2.4
K+
-
Bacillus subtilis
2.7.2.4
K+
-
Geobacillus stearothermophilus
2.7.2.4
K+
activity enhanced
Paenibacillus polymyxa
2.7.2.4
K+
-
Pseudomonas fluorescens
2.7.2.4
Mg2+
-
Neurospora crassa
2.7.2.4
Mg2+
-
Paenibacillus polymyxa
2.7.2.4
Mg2+
-
Saccharomyces cerevisiae
2.7.2.4
Mn2+
-
Neurospora crassa
2.7.2.4
Mn2+
-
Pseudomonas fluorescens
2.7.2.4
Mn2+
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.2.4
17000
-
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
2.7.2.4
43000
-
4 * 43000, aspartokinase II, equilibrium sedimentation
Escherichia coli
2.7.2.4
47000
-
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
2.7.2.4
60000
-
? * 60000, high-speed sedimentation equilibrium in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
66000
-
4 * 66000, ultracentrifugation
Escherichia coli
2.7.2.4
80000
-
4 * 80000, aspartokinase-homoserine dehydrogenase complex, sedimentation equlibrium performed on guanidinium chloride dissolved complex
Escherichia coli
2.7.2.4
84000
-
4 * 84000, SDS-PAGE
Escherichia coli
2.7.2.4
88000
-
4 * 88000, gel filtration in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
100000
-
gel filtration
Rhodocyclus tenuis
2.7.2.4
110000
-
gel filtration
Geobacillus stearothermophilus
2.7.2.4
116000
-
equilibrium ultracentrifugation
Paenibacillus polymyxa
2.7.2.4
122000
-
2 * 122000, ultracentrifugation in TES or HEPES buffer
Escherichia coli
2.7.2.4
125000
-
aspartokinase II
Bacillus subtilis
2.7.2.4
126000
-
gel filtration
Pseudomonas putida
2.7.2.4
127000
-
aspartokinase III, sedimentation equilibrium
Escherichia coli
2.7.2.4
169000
-
aspartokinase II, equilibrium sedimentation
Escherichia coli
2.7.2.4
250000
-
aspartokinase I
Bacillus subtilis
2.7.2.4
358000
-
light scattering studies
Escherichia coli
2.7.2.4
360000
-
equilibrium sedimentation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.4
ATP + L-aspartate
Salmonella enterica subsp. enterica serovar Typhimurium
-
ADP + 4-phospho-L-aspartate
-
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.2.4
ATP + L-aspartate
Escherichia coli
-
ADP + 4-phospho-L-aspartate
-
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
Saccharomyces cerevisiae
-
ADP + 4-phospho-L-aspartate
-
Saccharomyces cerevisiae
r
2.7.2.4
ATP + L-aspartate
Geobacillus stearothermophilus
-
ADP + 4-phospho-L-aspartate
-
Geobacillus stearothermophilus
r
2.7.2.4
ATP + L-aspartate
Neurospora crassa
-
ADP + 4-phospho-L-aspartate
-
Neurospora crassa
r
2.7.2.4
ATP + L-aspartate
Pseudomonas fluorescens
-
ADP + 4-phospho-L-aspartate
-
Pseudomonas fluorescens
r
2.7.2.4
ATP + L-aspartate
Rhodobacter capsulatus
-
ADP + 4-phospho-L-aspartate
-
Rhodobacter capsulatus
r
2.7.2.4
ATP + L-aspartate
Paenibacillus polymyxa
-
ADP + 4-phospho-L-aspartate
-
Paenibacillus polymyxa
r
2.7.2.4
ATP + L-aspartate
Rhodospirillum rubrum
-
ADP + 4-phospho-L-aspartate
-
Rhodospirillum rubrum
r
2.7.2.4
ATP + L-aspartate
Rhodocyclus tenuis
-
ADP + 4-phospho-L-aspartate
-
Rhodocyclus tenuis
r
2.7.2.4
ATP + L-aspartate
Bacillus subtilis
physiological role of aspartokinase II is to supply precursors for the amino acid pool
ADP + 4-phospho-L-aspartate
-
Bacillus subtilis
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.2.4
Azotobacter sp.
-
-
-
2.7.2.4
Bacillus cereus
-
-
-
2.7.2.4
Bacillus licheniformis
-
-
-
2.7.2.4
Bacillus subtilis
-
-
-
2.7.2.4
Brevibacterium flavum
-
-
-
2.7.2.4
Corynebacterium glutamicum
-
-
-
2.7.2.4
Escherichia coli
-
K12
-
2.7.2.4
Geobacillus stearothermophilus
-
-
-
2.7.2.4
Neurospora crassa
-
-
-
2.7.2.4
no activity in Edwardsiella sp.
-
-
-
2.7.2.4
no activity in Providencia sp.
-
-
-
2.7.2.4
Paenibacillus polymyxa
-
-
-
2.7.2.4
Pseudomonas aeruginosa
-
-
-
2.7.2.4
Pseudomonas fluorescens
-
-
-
2.7.2.4
Pseudomonas putida
-
-
-
2.7.2.4
Rhodobacter capsulatus
-
-
-
2.7.2.4
Rhodobacter sphaeroides
-
-
-
2.7.2.4
Rhodocyclus tenuis
-
-
-
2.7.2.4
Rhodospirillum rubrum
-
-
-
2.7.2.4
Saccharomyces cerevisiae
-
yeast
-
2.7.2.4
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.2.4
-
Paenibacillus polymyxa
2.7.2.4
-
Rhodobacter sphaeroides
2.7.2.4
-
Rhodocyclus tenuis
2.7.2.4
aspartokinase III
Escherichia coli
2.7.2.4
partially
Geobacillus stearothermophilus
Storage Stability
EC Number
Storage Stability
Organism
2.7.2.4
-10C, can be stored over a period of 6 months with a 40% loss of activity, longer storage does not lead to further inactivation
Paenibacillus polymyxa
2.7.2.4
-15C, aspartokinase II, stable in buffer containing 20% glycerol, remaining 100% active and homogenous for several months
Escherichia coli
2.7.2.4
25C, aspartokinase I, stable at room temperature either in presence of 1.0 mM L-threonine or of 0.15 M KCl
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.2.4
ATP + L-aspartate
-
642314
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 4-phospho-L-aspartate
-
642314
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.2.4
ATP + L-aspartate
-
642314
Bacillus subtilis
ADP + 4-phospho-L-aspartate
-
642314
Bacillus subtilis
r
2.7.2.4
ATP + L-aspartate
-
642314
Escherichia coli
ADP + 4-phospho-L-aspartate
-
642314
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
-
642314
Saccharomyces cerevisiae
ADP + 4-phospho-L-aspartate
-
642314
Saccharomyces cerevisiae
r
2.7.2.4
ATP + L-aspartate
-
642314
Geobacillus stearothermophilus
ADP + 4-phospho-L-aspartate
-
642314
Geobacillus stearothermophilus
r
2.7.2.4
ATP + L-aspartate
-
642314
Neurospora crassa
ADP + 4-phospho-L-aspartate
-
642314
Neurospora crassa
r
2.7.2.4
ATP + L-aspartate
-
642314
Pseudomonas fluorescens
ADP + 4-phospho-L-aspartate
-
642314
Pseudomonas fluorescens
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodobacter capsulatus
ADP + 4-phospho-L-aspartate
-
642314
Rhodobacter capsulatus
r
2.7.2.4
ATP + L-aspartate
-
642314
Paenibacillus polymyxa
ADP + 4-phospho-L-aspartate
-
642314
Paenibacillus polymyxa
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodospirillum rubrum
ADP + 4-phospho-L-aspartate
-
642314
Rhodospirillum rubrum
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodocyclus tenuis
ADP + 4-phospho-L-aspartate
-
-
-
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodocyclus tenuis
ADP + 4-phospho-L-aspartate
-
642314
Rhodocyclus tenuis
r
2.7.2.4
ATP + L-aspartate
maximum velocity of the reverse reaction is only one-twelfth that of the forward reaction, but has the advantage of using commercial substrates
642314
Escherichia coli
ADP + 4-phospho-L-aspartate
-
642314
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
physiological role of aspartokinase II is to supply precursors for the amino acid pool
642314
Bacillus subtilis
ADP + 4-phospho-L-aspartate
-
642314
Bacillus subtilis
r
2.7.2.4
additional information
-
642314
Escherichia coli
?
-
-
-
-
2.7.2.4
additional information
-
642314
Saccharomyces cerevisiae
?
-
-
-
-
2.7.2.4
additional information
-
642314
Pseudomonas fluorescens
?
-
-
-
-
2.7.2.4
additional information
-
642314
Paenibacillus polymyxa
?
-
-
-
-
2.7.2.4
additional information
no other natural aminoacids or D-aspartate are substrates of this reaction
642314
Neurospora crassa
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.2.4
dimer
2 * 122000, ultracentrifugation in TES or HEPES buffer
Escherichia coli
2.7.2.4
heterodimer
-
Bacillus subtilis
2.7.2.4
oligomer
? * 60000, high-speed sedimentation equilibrium in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
tetramer
4 * 43000, aspartokinase II, equilibrium sedimentation; 4 * 66000, ultracentrifugation; 4 * 80000-120000, sedimentation in sucrose gradient in absence of threonine; 4 * 80000, aspartokinase-homoserine dehydrogenase complex, sedimentation equlibrium performed on guanidinium chloride dissolved complex; 4 * 84000, SDS-PAGE; 4 * 88000, gel filtration in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
tetramer
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.2.4
55
-
-
Geobacillus stearothermophilus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.2.4
14.2
-
L-aspartate
aspartokinase II
Escherichia coli
2.7.2.4
39.2
-
L-aspartate
aspartokinase III
Escherichia coli
2.7.2.4
56.7
-
L-aspartate
aspartokinase I
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.2.4
8
-
-
Geobacillus stearothermophilus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.2.4
6
9.5
aspartokinase I, pH range for 50% activity
Bacillus subtilis
2.7.2.4
6.5
8.2
aspartokinase II, pH range for 50% activity
Bacillus subtilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.2.4
NH4+
-
Geobacillus stearothermophilus
2.7.2.4
NH4+
-
Paenibacillus polymyxa
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.2.4
L-lysine, L-threonine or L-methionine protects the enzymic activity against heat inactivation
Rhodocyclus tenuis
2.7.2.4
nonpolar L-amino acids protect from inactivation by heat and detergent and reverse the inhibition caused by feedback inhibitors L-lysine and L-threonine
Paenibacillus polymyxa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.2.4
aspartate-beta-semialdehyde
-
Bacillus licheniformis
2.7.2.4
aspartate-beta-semialdehyde
-
Rhodobacter sphaeroides
2.7.2.4
DL-meso-diaminopimelic acid
aspartokinase I, noncompetitive inhibition
Bacillus subtilis
2.7.2.4
DL-meso-diaminopimelic acid
-
Geobacillus stearothermophilus
2.7.2.4
L-lysine
-
Azotobacter sp.
2.7.2.4
L-lysine
-
Bacillus cereus
2.7.2.4
L-lysine
-
Bacillus licheniformis
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Brevibacterium flavum
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Corynebacterium glutamicum
2.7.2.4
L-lysine
-
Geobacillus stearothermophilus
2.7.2.4
L-lysine
-
Paenibacillus polymyxa
2.7.2.4
L-lysine
-
Pseudomonas aeruginosa
2.7.2.4
L-lysine
concerted feedback inhibition with L-threonine
Pseudomonas fluorescens
2.7.2.4
L-lysine
-
Pseudomonas putida
2.7.2.4
L-lysine
-
Rhodobacter capsulatus
2.7.2.4
L-lysine
-
Rhodobacter sphaeroides
2.7.2.4
L-lysine
-
Rhodocyclus tenuis
2.7.2.4
L-lysine
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.4
L-lysine
-
Bacillus subtilis
2.7.2.4
L-lysine
-
Escherichia coli
2.7.2.4
L-threonine
aspartokinase II, competitive inhibition
Bacillus subtilis
2.7.2.4
L-threonine
-
Geobacillus stearothermophilus
2.7.2.4
L-threonine
-
Paenibacillus polymyxa
2.7.2.4
L-threonine
-
Pseudomonas aeruginosa
2.7.2.4
L-threonine
-
Pseudomonas fluorescens
2.7.2.4
L-threonine
-
Pseudomonas putida
2.7.2.4
L-threonine
-
Rhodocyclus tenuis
2.7.2.4
L-threonine
-
Saccharomyces cerevisiae
2.7.2.4
L-threonine
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.18
-
ATP
aspartokinase I
Escherichia coli
2.7.2.4
0.9
-
L-aspartate
-
Rhodocyclus tenuis
2.7.2.4
1.5
-
L-aspartate
aspartokinase I
Escherichia coli
2.7.2.4
1.5
-
L-aspartate
-
Paenibacillus polymyxa
2.7.2.4
1.9
-
ATP
aspartokinase II, 27C
Escherichia coli
2.7.2.4
2.1
-
L-aspartate
aspartokinase II, 27C
Escherichia coli
2.7.2.4
3
-
ATP
-
Rhodocyclus tenuis
2.7.2.4
3
-
L-aspartate
aspartokinase I
Bacillus subtilis
2.7.2.4
4
-
ATP
aspartokinase I
Escherichia coli
2.7.2.4
4.7
-
L-aspartate
aspartokinase III, 27C
Escherichia coli
2.7.2.4
4.8
-
ATP
aspartokinase III, 27C
Escherichia coli
2.7.2.4
4.8
-
ATP
-
Pseudomonas putida
2.7.2.4
4.8
-
L-aspartate
-
Pseudomonas putida
2.7.2.4
17
-
L-aspartate
aspartokinase II
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.2.4
Fe2+
-
Neurospora crassa
2.7.2.4
Fe2+
-
Paenibacillus polymyxa
2.7.2.4
Fe2+
-
Saccharomyces cerevisiae
2.7.2.4
K+
-
Bacillus subtilis
2.7.2.4
K+
-
Geobacillus stearothermophilus
2.7.2.4
K+
activity enhanced
Paenibacillus polymyxa
2.7.2.4
K+
-
Pseudomonas fluorescens
2.7.2.4
Mg2+
-
Neurospora crassa
2.7.2.4
Mg2+
-
Paenibacillus polymyxa
2.7.2.4
Mg2+
-
Saccharomyces cerevisiae
2.7.2.4
Mn2+
-
Neurospora crassa
2.7.2.4
Mn2+
-
Pseudomonas fluorescens
2.7.2.4
Mn2+
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.2.4
17000
-
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
2.7.2.4
43000
-
4 * 43000, aspartokinase II, equilibrium sedimentation
Escherichia coli
2.7.2.4
47000
-
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
2.7.2.4
60000
-
? * 60000, high-speed sedimentation equilibrium in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
66000
-
4 * 66000, ultracentrifugation
Escherichia coli
2.7.2.4
80000
-
4 * 80000, aspartokinase-homoserine dehydrogenase complex, sedimentation equlibrium performed on guanidinium chloride dissolved complex
Escherichia coli
2.7.2.4
84000
-
4 * 84000, SDS-PAGE
Escherichia coli
2.7.2.4
88000
-
4 * 88000, gel filtration in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
100000
-
gel filtration
Rhodocyclus tenuis
2.7.2.4
110000
-
gel filtration
Geobacillus stearothermophilus
2.7.2.4
116000
-
equilibrium ultracentrifugation
Paenibacillus polymyxa
2.7.2.4
122000
-
2 * 122000, ultracentrifugation in TES or HEPES buffer
Escherichia coli
2.7.2.4
125000
-
aspartokinase II
Bacillus subtilis
2.7.2.4
126000
-
gel filtration
Pseudomonas putida
2.7.2.4
127000
-
aspartokinase III, sedimentation equilibrium
Escherichia coli
2.7.2.4
169000
-
aspartokinase II, equilibrium sedimentation
Escherichia coli
2.7.2.4
250000
-
aspartokinase I
Bacillus subtilis
2.7.2.4
358000
-
light scattering studies
Escherichia coli
2.7.2.4
360000
-
equilibrium sedimentation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.4
ATP + L-aspartate
Salmonella enterica subsp. enterica serovar Typhimurium
-
ADP + 4-phospho-L-aspartate
-
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.2.4
ATP + L-aspartate
Escherichia coli
-
ADP + 4-phospho-L-aspartate
-
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
Saccharomyces cerevisiae
-
ADP + 4-phospho-L-aspartate
-
Saccharomyces cerevisiae
r
2.7.2.4
ATP + L-aspartate
Geobacillus stearothermophilus
-
ADP + 4-phospho-L-aspartate
-
Geobacillus stearothermophilus
r
2.7.2.4
ATP + L-aspartate
Neurospora crassa
-
ADP + 4-phospho-L-aspartate
-
Neurospora crassa
r
2.7.2.4
ATP + L-aspartate
Pseudomonas fluorescens
-
ADP + 4-phospho-L-aspartate
-
Pseudomonas fluorescens
r
2.7.2.4
ATP + L-aspartate
Rhodobacter capsulatus
-
ADP + 4-phospho-L-aspartate
-
Rhodobacter capsulatus
r
2.7.2.4
ATP + L-aspartate
Paenibacillus polymyxa
-
ADP + 4-phospho-L-aspartate
-
Paenibacillus polymyxa
r
2.7.2.4
ATP + L-aspartate
Rhodospirillum rubrum
-
ADP + 4-phospho-L-aspartate
-
Rhodospirillum rubrum
r
2.7.2.4
ATP + L-aspartate
Rhodocyclus tenuis
-
ADP + 4-phospho-L-aspartate
-
Rhodocyclus tenuis
r
2.7.2.4
ATP + L-aspartate
Bacillus subtilis
physiological role of aspartokinase II is to supply precursors for the amino acid pool
ADP + 4-phospho-L-aspartate
-
Bacillus subtilis
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.2.4
-
Paenibacillus polymyxa
2.7.2.4
-
Rhodobacter sphaeroides
2.7.2.4
-
Rhodocyclus tenuis
2.7.2.4
aspartokinase III
Escherichia coli
2.7.2.4
partially
Geobacillus stearothermophilus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.2.4
-10C, can be stored over a period of 6 months with a 40% loss of activity, longer storage does not lead to further inactivation
Paenibacillus polymyxa
2.7.2.4
-15C, aspartokinase II, stable in buffer containing 20% glycerol, remaining 100% active and homogenous for several months
Escherichia coli
2.7.2.4
25C, aspartokinase I, stable at room temperature either in presence of 1.0 mM L-threonine or of 0.15 M KCl
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.2.4
ATP + L-aspartate
-
642314
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 4-phospho-L-aspartate
-
642314
Salmonella enterica subsp. enterica serovar Typhimurium
r
2.7.2.4
ATP + L-aspartate
-
642314
Bacillus subtilis
ADP + 4-phospho-L-aspartate
-
642314
Bacillus subtilis
r
2.7.2.4
ATP + L-aspartate
-
642314
Escherichia coli
ADP + 4-phospho-L-aspartate
-
642314
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
-
642314
Saccharomyces cerevisiae
ADP + 4-phospho-L-aspartate
-
642314
Saccharomyces cerevisiae
r
2.7.2.4
ATP + L-aspartate
-
642314
Geobacillus stearothermophilus
ADP + 4-phospho-L-aspartate
-
642314
Geobacillus stearothermophilus
r
2.7.2.4
ATP + L-aspartate
-
642314
Neurospora crassa
ADP + 4-phospho-L-aspartate
-
642314
Neurospora crassa
r
2.7.2.4
ATP + L-aspartate
-
642314
Pseudomonas fluorescens
ADP + 4-phospho-L-aspartate
-
642314
Pseudomonas fluorescens
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodobacter capsulatus
ADP + 4-phospho-L-aspartate
-
642314
Rhodobacter capsulatus
r
2.7.2.4
ATP + L-aspartate
-
642314
Paenibacillus polymyxa
ADP + 4-phospho-L-aspartate
-
642314
Paenibacillus polymyxa
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodospirillum rubrum
ADP + 4-phospho-L-aspartate
-
642314
Rhodospirillum rubrum
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodocyclus tenuis
ADP + 4-phospho-L-aspartate
-
-
-
r
2.7.2.4
ATP + L-aspartate
-
642314
Rhodocyclus tenuis
ADP + 4-phospho-L-aspartate
-
642314
Rhodocyclus tenuis
r
2.7.2.4
ATP + L-aspartate
maximum velocity of the reverse reaction is only one-twelfth that of the forward reaction, but has the advantage of using commercial substrates
642314
Escherichia coli
ADP + 4-phospho-L-aspartate
-
642314
Escherichia coli
r
2.7.2.4
ATP + L-aspartate
physiological role of aspartokinase II is to supply precursors for the amino acid pool
642314
Bacillus subtilis
ADP + 4-phospho-L-aspartate
-
642314
Bacillus subtilis
r
2.7.2.4
additional information
-
642314
Escherichia coli
?
-
-
-
-
2.7.2.4
additional information
-
642314
Saccharomyces cerevisiae
?
-
-
-
-
2.7.2.4
additional information
-
642314
Pseudomonas fluorescens
?
-
-
-
-
2.7.2.4
additional information
-
642314
Paenibacillus polymyxa
?
-
-
-
-
2.7.2.4
additional information
no other natural aminoacids or D-aspartate are substrates of this reaction
642314
Neurospora crassa
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.2.4
dimer
2 * 122000, ultracentrifugation in TES or HEPES buffer
Escherichia coli
2.7.2.4
heterodimer
-
Bacillus subtilis
2.7.2.4
oligomer
? * 60000, high-speed sedimentation equilibrium in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
tetramer
4 * 43000, aspartokinase II, equilibrium sedimentation; 4 * 66000, ultracentrifugation; 4 * 80000-120000, sedimentation in sucrose gradient in absence of threonine; 4 * 80000, aspartokinase-homoserine dehydrogenase complex, sedimentation equlibrium performed on guanidinium chloride dissolved complex; 4 * 84000, SDS-PAGE; 4 * 88000, gel filtration in 6.0 mM guanidinium chloride
Escherichia coli
2.7.2.4
tetramer
2 * 17000 + 2 * 47000, SDS-PAGE
Paenibacillus polymyxa
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.2.4
55
-
-
Geobacillus stearothermophilus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.2.4
14.2
-
L-aspartate
aspartokinase II
Escherichia coli
2.7.2.4
39.2
-
L-aspartate
aspartokinase III
Escherichia coli
2.7.2.4
56.7
-
L-aspartate
aspartokinase I
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.2.4
8
-
-
Geobacillus stearothermophilus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.2.4
6
9.5
aspartokinase I, pH range for 50% activity
Bacillus subtilis
2.7.2.4
6.5
8.2
aspartokinase II, pH range for 50% activity
Bacillus subtilis