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Literature summary extracted from

  • Kovari, Z.; Flachner, B.; Naray-Szabo, G.; Vas, M.
    Crystallographic and thiol-reactivity studies on the complex of pig muscle phosphoglycerate kinase with ATP analogues: correlation between nucleotide binding mode and helix flexibility (2002), Biochemistry, 41, 8796-8806.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 enzyme in ternary complex with Mg2+-bound ATP analogue inhibitors Mg-beta,gamma-methylene-adenosine-5'-triphosphate and Mg-beta,gamma-imido-adenosine-5'-triphosphate, and substrate 3-phospho-D-glycerate, hanging drop vapour diffusion method, reservoir solution: 10 mM beta,gamma-methylene-adenosine-5'-triphosphate, 12 mM MgCl2, 10 mM 3-phospho-D-glycerate, 27-28% w/w polyethylene glycol 8000, pH 7.0, 15°C, a few weeks, X-ray structure determination and analysis Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid) nucleotide ligands protect, e.g. MgATP2-, MgADP-, Mg-beta,gamma-methylene-adenosine-5'-triphosphate, Mg-beta,gamma-imido-adenosine-5'-triphosphate Sus scrofa
2.7.2.3 beta,gamma-imido-adenosine-5'-triphosphate binding structure; i.e. AMP-PNP, an ATP analogue Sus scrofa
2.7.2.3 beta,gamma-methylene-adenosine-5'-triphosphate binding structure; i.e. AMP-PCP, an ATP analogue Sus scrofa
2.7.2.3 iodoacetamide nucleotide ligands protect, e.g. MgATP2-, MgADP-, Mg-beta,gamma-methylene-adenosine-5'-triphosphate, Mg-beta,gamma-imido-adenosine-5'-triphosphate Sus scrofa
2.7.2.3 Mg-beta,gamma-imido-adenosine-5'-triphosphate
-
 Sus scrofa
2.7.2.3 Mg-beta,gamma-methylene-adenosine-5'-triphosphate
-
 Sus scrofa
2.7.2.3 MgADP-
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Sus scrofa Q7SIB7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 muscle
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Sus scrofa ADP + 1,3-diphosphoglycerate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Sus scrofa
2.7.2.3 ATP required as phosphate donor Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.3 0.039
-
 MgADP- pH 7.5, 20°C Sus scrofa
2.7.2.3 0.58
-
 Mg-beta,gamma-imido-adenosine-5'-triphosphate pH 7.5, 20°C Sus scrofa
2.7.2.3 1.21
-
 Mg-beta,gamma-methylene-adenosine-5'-triphosphate pH 7.5, 20°C Sus scrofa