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Literature summary extracted from

  • Nojima, H.; Oshima, T.; Noda, H.
    Purification and properties of phosphoglycerate kinase from Thermus thermophilus strain HB8 (1979), J. Biochem., 85, 1509-1517.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 crystallization from ammonium sulfate precipitation overnight with gradually raising of temperature from 4°C to room temperature, in presence of ATP Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 (NH4)2SO4 strong Thermus thermophilus
2.7.2.3 2,3-diphosphoglycerate
-
 Thermus thermophilus
2.7.2.3 ADP
-
 Thermus thermophilus
2.7.2.3 CTP competitive to ATP Thermus thermophilus
2.7.2.3 GTP competitive to ATP Thermus thermophilus
2.7.2.3 ITP weak competitive inhibitor Thermus thermophilus
2.7.2.3 NaCl weak Thermus thermophilus
2.7.2.3 UTP weak Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.28
-
 ATP pH 7.5, 22°C Thermus thermophilus
2.7.2.3 1.79
-
 3-phospho-D-glycerate pH 7.5, 22°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Mg2+ required Thermus thermophilus
2.7.2.3 Mg2+ optimum concentration: 8-16 mM Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 44000
-
 1 * 44000, SDS-PAGE Thermus thermophilus
2.7.2.3 44600
-
 sedimentation equilibrium studies Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Thermus thermophilus
-
-
-
2.7.2.3 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 to homogeneity Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 259
-
 purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate high specificity for ATP Thermus thermophilus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate high specificity for ATP Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 CTP + 3-phospho-D-glycerate low activity Thermus thermophilus CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate low activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate low activity Thermus thermophilus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate low activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Thermus thermophilus IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate no activity Thermus thermophilus UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate no activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 UDP + 1,3-diphosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 44000, SDS-PAGE Thermus thermophilus
2.7.2.3 More secondary structure: 29% alpha-helix, 11% pleated sheets Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 22
-
 assay at Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 79
-
 20 min, no loss of activity Thermus thermophilus
2.7.2.3 92
-
 5 min, 75% loss of activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 6 8.5
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.3 4.8 10 pH 4.8: about 45% of activity maximum, pH 10.0: about 90% of activity maximum Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Thermus thermophilus
2.7.2.3 ATP required as phosphate donor Thermus thermophilus
2.7.2.3 CTP
-
 Thermus thermophilus
2.7.2.3 GTP
-
 Thermus thermophilus
2.7.2.3 ITP
-
 Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.3 Thermus thermophilus analytical electric focusing
-
 5