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Literature summary extracted from

  • Fifis, T.; Scopes, R.K.
    Purification of 3-phosphoglycerate kinase from diverse sources by affinity elution chromatography (1978), Biochem. J., 175, 311-319.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information
-
 Escherichia coli
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Gallus gallus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Beta vulgaris
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Rattus norvegicus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Sus scrofa
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Saccharomyces cerevisiae
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Bos taurus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Oryctolagus cuniculus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Ovis aries
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Spinacia oleracea
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Equus caballus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Cyprinus carpio
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Oncorhynchus mykiss
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Columba sp.
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Salmo trutta
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Trichosurus vulpecula
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Macropus giganteus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Macropus fuliginosus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Thylogale billardierii
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Pseudocheirus peregrinus
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Bufo vulgaris
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Dromaius novaehollandiae
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Abramis brama
2.7.2.3 additional information
-
 additional information Km-value for MgATP, activation energy Chrysophrys guttulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 45000 48000 gel filtration Gallus gallus
2.7.2.3 45000 48000 gel filtration Beta vulgaris
2.7.2.3 45000 48000 gel filtration Rattus norvegicus
2.7.2.3 45000 48000 gel filtration Sus scrofa
2.7.2.3 45000 48000 gel filtration Saccharomyces cerevisiae
2.7.2.3 45000 48000 gel filtration Bos taurus
2.7.2.3 45000 48000 gel filtration Oryctolagus cuniculus
2.7.2.3 45000 48000 gel filtration Spinacia oleracea
2.7.2.3 45000 48000 gel filtration Equus caballus
2.7.2.3 45000 48000 gel filtration Cyprinus carpio
2.7.2.3 45000 48000 gel filtration Oncorhynchus mykiss
2.7.2.3 45000 48000 gel filtration Columba sp.
2.7.2.3 45000 48000 gel filtration Salmo trutta
2.7.2.3 45000 48000 gel filtration Trichosurus vulpecula
2.7.2.3 45000 48000 gel filtration Macropus giganteus
2.7.2.3 45000 48000 gel filtration Macropus fuliginosus
2.7.2.3 45000 48000 gel filtration Thylogale billardierii
2.7.2.3 45000 48000 gel filtration Pseudocheirus peregrinus
2.7.2.3 45000 48000 gel filtration Bufo vulgaris
2.7.2.3 45000 48000 gel filtration Dromaius novaehollandiae
2.7.2.3 45000 48000 gel filtration Abramis brama
2.7.2.3 45000 48000 gel filtration Chrysophrys guttulatus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Abramis brama
-
 bream
-
2.7.2.3 Beta vulgaris
-
 silver beet
-
2.7.2.3 Bos taurus
-
 ox
-
2.7.2.3 Bufo vulgaris
-
 toad
-
2.7.2.3 Chrysophrys guttulatus
-
 red snapper
-
2.7.2.3 Columba sp.
-
-
-
2.7.2.3 Cyprinus carpio
-
 carp
-
2.7.2.3 Dromaius novaehollandiae
-
 emu
-
2.7.2.3 Equus caballus
-
-
-
2.7.2.3 Escherichia coli
-
-
-
2.7.2.3 Gallus gallus
-
-
-
2.7.2.3 Macropus fuliginosus
-
 Western grey kangaroo
-
2.7.2.3 Macropus giganteus
-
 Eastern grey kangaroo
-
2.7.2.3 Oncorhynchus mykiss
-
-
-
2.7.2.3 Oryctolagus cuniculus
-
-
-
2.7.2.3 Ovis aries
-
-
-
2.7.2.3 Pseudocheirus peregrinus
-
 ringtail possum
-
2.7.2.3 Rattus norvegicus
-
-
-
2.7.2.3 Saccharomyces cerevisiae
-
-
-
2.7.2.3 Salmo trutta
-
-
-
2.7.2.3 Spinacia oleracea
-
-
-
2.7.2.3 Sus scrofa
-
-
-
2.7.2.3 Thylogale billardierii
-
-
-
2.7.2.3 Trichosurus vulpecula
-
 brushtail possum
-
2.7.2.3 Trichosurus vulpecula
-
 vulepeculus
-
2.7.2.3 Vombatus ursinus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Gallus gallus
2.7.2.3
-
 Beta vulgaris
2.7.2.3
-
 Escherichia coli
2.7.2.3
-
 Saccharomyces cerevisiae
2.7.2.3
-
 Ovis aries
2.7.2.3
-
 Spinacia oleracea
2.7.2.3
-
 Equus caballus
2.7.2.3
-
 Columba sp.
2.7.2.3
-
 Salmo trutta
2.7.2.3
-
 Trichosurus vulpecula
2.7.2.3
-
 Vombatus ursinus
2.7.2.3
-
 Macropus giganteus
2.7.2.3
-
 Macropus fuliginosus
2.7.2.3
-
 Thylogale billardierii
2.7.2.3
-
 Pseudocheirus peregrinus
2.7.2.3
-
 Bufo vulgaris
2.7.2.3
-
 Dromaius novaehollandiae
2.7.2.3
-
 Abramis brama
2.7.2.3
-
 Chrysophrys guttulatus
2.7.2.3 from liver and muscle Bos taurus
2.7.2.3 from muscle and liver Rattus norvegicus
2.7.2.3 from muscle and liver Oryctolagus cuniculus
2.7.2.3 purification from diverse sources by affinity elution chromatography Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 leaf
-
 Beta vulgaris
-
2.7.2.3 leaf
-
 Spinacia oleracea
-
2.7.2.3 liver
-
 Rattus norvegicus
-
2.7.2.3 liver
-
 Bos taurus
-
2.7.2.3 liver
-
 Oryctolagus cuniculus
-
2.7.2.3 muscle
-
 Gallus gallus
-
2.7.2.3 muscle
-
 Rattus norvegicus
-
2.7.2.3 muscle
-
 Sus scrofa
-
2.7.2.3 muscle
-
 Bos taurus
-
2.7.2.3 muscle
-
 Oryctolagus cuniculus
-
2.7.2.3 muscle
-
 Ovis aries
-
2.7.2.3 muscle
-
 Equus caballus
-
2.7.2.3 muscle
-
 Cyprinus carpio
-
2.7.2.3 muscle
-
 Oncorhynchus mykiss
-
2.7.2.3 muscle
-
 Columba sp.
-
2.7.2.3 muscle
-
 Salmo trutta
-
2.7.2.3 muscle
-
 Trichosurus vulpecula
-
2.7.2.3 muscle
-
 Vombatus ursinus
-
2.7.2.3 muscle
-
 Macropus giganteus
-
2.7.2.3 muscle
-
 Macropus fuliginosus
-
2.7.2.3 muscle
-
 Thylogale billardierii
-
2.7.2.3 muscle
-
 Pseudocheirus peregrinus
-
2.7.2.3 muscle
-
 Bufo vulgaris
-
2.7.2.3 muscle
-
 Dromaius novaehollandiae
-
2.7.2.3 muscle
-
 Abramis brama
-
2.7.2.3 muscle
-
 Chrysophrys guttulatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 300
-
 purified liver enzyme Rattus norvegicus
2.7.2.3 460
-
 purified muscle enzyme Rattus norvegicus
2.7.2.3 470
-
 purified enzyme Bufo vulgaris
2.7.2.3 480
-
 purified enzyme Escherichia coli
2.7.2.3 540
-
 purified liver enzyme Oryctolagus cuniculus
2.7.2.3 560
-
 purified enzyme Abramis brama
2.7.2.3 600
-
 purified enzyme Macropus giganteus
2.7.2.3 620
-
 purified enzyme Columba sp.
2.7.2.3 650
-
 purified enzyme Macropus fuliginosus
2.7.2.3 650
-
 purified liver enzyme Bos taurus
2.7.2.3 680
-
 purified enzyme Gallus gallus
2.7.2.3 680
-
 purified enzyme Ovis aries
2.7.2.3 690
-
 purified enzyme Salmo trutta
2.7.2.3 690
-
 purified enzyme Pseudocheirus peregrinus
2.7.2.3 690
-
 purified enzyme Chrysophrys guttulatus
2.7.2.3 700
-
-
 Ovis aries
2.7.2.3 700
-
 purified enzyme Sus scrofa
2.7.2.3 700
-
 purified muscle enzyme Saccharomyces cerevisiae
2.7.2.3 700
-
 purified muscle enzyme Oryctolagus cuniculus
2.7.2.3 700
-
 purified muscle enzyme Equus caballus
2.7.2.3 700
-
 purified muscle enzyme Trichosurus vulpecula
2.7.2.3 700
-
 purified muscle enzyme Vombatus ursinus
2.7.2.3 710
-
 purified muscle enzyme Bos taurus
2.7.2.3 720
-
-
 Ovis aries
2.7.2.3 720
-
 purified enzyme Oncorhynchus mykiss
2.7.2.3 720
-
 purified enzyme Dromaius novaehollandiae
2.7.2.3 780
-
 purified enzyme Beta vulgaris
2.7.2.3 780
-
 purified enzyme Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Gallus gallus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Beta vulgaris ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Escherichia coli ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Rattus norvegicus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Sus scrofa ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Bos taurus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Oryctolagus cuniculus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Ovis aries ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Spinacia oleracea ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Equus caballus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Cyprinus carpio ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Oncorhynchus mykiss ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Columba sp. ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Salmo trutta ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Trichosurus vulpecula ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Vombatus ursinus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Macropus giganteus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Macropus fuliginosus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Thylogale billardierii ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Pseudocheirus peregrinus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Bufo vulgaris ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Dromaius novaehollandiae ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Abramis brama ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Chrysophrys guttulatus ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer
-
 Ovis aries
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Gallus gallus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Beta vulgaris
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Rattus norvegicus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Sus scrofa
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Saccharomyces cerevisiae
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Bos taurus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Oryctolagus cuniculus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Spinacia oleracea
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Equus caballus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Cyprinus carpio
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Oncorhynchus mykiss
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Columba sp.
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Salmo trutta
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Trichosurus vulpecula
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Macropus giganteus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Macropus fuliginosus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Thylogale billardierii
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Pseudocheirus peregrinus
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Bufo vulgaris
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Dromaius novaehollandiae
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Abramis brama
2.7.2.3 monomer 1 * 45000-48000, SDS-PAGE Chrysophrys guttulatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Gallus gallus
2.7.2.3 ADP
-
 Beta vulgaris
2.7.2.3 ADP
-
 Escherichia coli
2.7.2.3 ADP
-
 Rattus norvegicus
2.7.2.3 ADP
-
 Sus scrofa
2.7.2.3 ADP
-
 Saccharomyces cerevisiae
2.7.2.3 ADP
-
 Bos taurus
2.7.2.3 ADP
-
 Oryctolagus cuniculus
2.7.2.3 ADP
-
 Ovis aries
2.7.2.3 ADP
-
 Spinacia oleracea
2.7.2.3 ADP
-
 Equus caballus
2.7.2.3 ADP
-
 Cyprinus carpio
2.7.2.3 ADP
-
 Oncorhynchus mykiss
2.7.2.3 ADP
-
 Columba sp.
2.7.2.3 ADP
-
 Salmo trutta
2.7.2.3 ADP
-
 Trichosurus vulpecula
2.7.2.3 ADP
-
 Vombatus ursinus
2.7.2.3 ADP
-
 Macropus giganteus
2.7.2.3 ADP
-
 Macropus fuliginosus
2.7.2.3 ADP
-
 Thylogale billardierii
2.7.2.3 ADP
-
 Pseudocheirus peregrinus
2.7.2.3 ADP
-
 Bufo vulgaris
2.7.2.3 ADP
-
 Dromaius novaehollandiae
2.7.2.3 ADP
-
 Abramis brama
2.7.2.3 ADP
-
 Chrysophrys guttulatus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Gallus gallus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Beta vulgaris
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Escherichia coli
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Sus scrofa
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Saccharomyces cerevisiae
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Bos taurus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Oryctolagus cuniculus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Ovis aries
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Spinacia oleracea
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Equus caballus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Cyprinus carpio
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Oncorhynchus mykiss
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Columba sp.
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Salmo trutta
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Trichosurus vulpecula
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Macropus giganteus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Macropus fuliginosus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Thylogale billardierii
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Pseudocheirus peregrinus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Bufo vulgaris
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Dromaius novaehollandiae
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Abramis brama
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Chrysophrys guttulatus
2.7.2.3 ATP required as phosphate donor Gallus gallus
2.7.2.3 ATP required as phosphate donor Beta vulgaris
2.7.2.3 ATP required as phosphate donor Escherichia coli
2.7.2.3 ATP required as phosphate donor Rattus norvegicus
2.7.2.3 ATP required as phosphate donor Sus scrofa
2.7.2.3 ATP required as phosphate donor Saccharomyces cerevisiae
2.7.2.3 ATP required as phosphate donor Bos taurus
2.7.2.3 ATP required as phosphate donor Oryctolagus cuniculus
2.7.2.3 ATP required as phosphate donor Ovis aries
2.7.2.3 ATP required as phosphate donor Spinacia oleracea
2.7.2.3 ATP required as phosphate donor Equus caballus
2.7.2.3 ATP required as phosphate donor Cyprinus carpio
2.7.2.3 ATP required as phosphate donor Oncorhynchus mykiss
2.7.2.3 ATP required as phosphate donor Columba sp.
2.7.2.3 ATP required as phosphate donor Salmo trutta
2.7.2.3 ATP required as phosphate donor Trichosurus vulpecula
2.7.2.3 ATP required as phosphate donor Vombatus ursinus
2.7.2.3 ATP required as phosphate donor Macropus giganteus
2.7.2.3 ATP required as phosphate donor Macropus fuliginosus
2.7.2.3 ATP required as phosphate donor Thylogale billardierii
2.7.2.3 ATP required as phosphate donor Pseudocheirus peregrinus
2.7.2.3 ATP required as phosphate donor Bufo vulgaris
2.7.2.3 ATP required as phosphate donor Dromaius novaehollandiae
2.7.2.3 ATP required as phosphate donor Abramis brama
2.7.2.3 ATP required as phosphate donor Chrysophrys guttulatus