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Literature summary extracted from

  • Suzuki, K.; Imahori, K.
    Phosphoglycerate kinase from Bacillus stearothermophilus (1982), Methods Enzymol., 90, 126-130.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 Co2+ 58% Geobacillus stearothermophilus
2.7.2.3 nucleoside diphosphates purine nucleotide diphosphates Geobacillus stearothermophilus
2.7.2.3 nucleoside monophosphates purine nucleotide monophosphates Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 2.2
-
 3-phospho-D-glycerate pH 7.6, 25°C Geobacillus stearothermophilus
2.7.2.3 2.9
-
 ATP pH 7.6, 25°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Ca2+ can partially replace Mg2+ in activation Geobacillus stearothermophilus
2.7.2.3 Ca2+ 15% of the activity with Mg2+ Geobacillus stearothermophilus
2.7.2.3 Co2+ can partially replace Mg2+ in activation Geobacillus stearothermophilus
2.7.2.3 Co2+ 58% of the activity with Mg2+ Geobacillus stearothermophilus
2.7.2.3 divalent cation Mg2+ or Mn2+ Geobacillus stearothermophilus
2.7.2.3 Mg2+
-
 Geobacillus stearothermophilus
2.7.2.3 Mn2+ Mn2+ or Mg2+ required Geobacillus stearothermophilus
2.7.2.3 additional information no activation by Ba2+ Geobacillus stearothermophilus
2.7.2.3 Ni2+ no activation Geobacillus stearothermophilus
2.7.2.3 Zn2+ no activation Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 42000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Geobacillus stearothermophilus
-
 NCA 1503
-
2.7.2.3 Geobacillus stearothermophilus NCA 1503
-
 NCA 1503
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 557
-
 purified enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate absolute specificity for 3-phospho-D-glycerate Geobacillus stearothermophilus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate absolute specificity for 3-phospho-D-glycerate Geobacillus stearothermophilus NCA 1503 ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 GTP + 3-phospho-D-glycerate 27% of the activity with ATP Geobacillus stearothermophilus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate 27% of the activity with ATP Geobacillus stearothermophilus NCA 1503 GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate 42% of the activity with ATP Geobacillus stearothermophilus IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate 42% of the activity with ATP Geobacillus stearothermophilus NCA 1503 IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 additional information no activity with pyrimidine nucleotides Geobacillus stearothermophilus ?
-
 ?
2.7.2.3 additional information no activity with pyrimidine nucleotides Geobacillus stearothermophilus NCA 1503 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 42000, SDS-PAGE Geobacillus stearothermophilus
2.7.2.3 More secondary structure: 20% alpha-helix, 45% beta-structure Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Geobacillus stearothermophilus
2.7.2.3 55 60 physiological temperature Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 60
-
 10 min, stable Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 5.5 8.5
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.3 5.5
-
 inactivation below Geobacillus stearothermophilus
2.7.2.3 6
-
 stable above Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Geobacillus stearothermophilus
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Geobacillus stearothermophilus
2.7.2.3 ATP required as phosphate donor Geobacillus stearothermophilus
2.7.2.3 GTP
-
 Geobacillus stearothermophilus
2.7.2.3 ITP
-
 Geobacillus stearothermophilus