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Literature summary extracted from

  • Kuntz, G.W.K.; Krietsch, W.K.G.
    Phosphoglycerate kinase from spinach, blue-green algae, and yeast (1982), Methods Enzymol., 90, 110-114.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Saccharomyces cerevisiae
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.2
-
 ADP ADP in form of MgADP- Saccharomyces cerevisiae
2.7.2.3 0.27
-
 ADP ADP in form of MgADP- Spinacia oleracea
2.7.2.3 0.3
-
 ATP ATP in form of MgATP2- Spinacia oleracea
2.7.2.3 0.48
-
 ATP ATP in form of MgATP2- Saccharomyces cerevisiae
2.7.2.3 0.48
-
 ATP ATP in form of MgATP2- Spirulina geitleri
2.7.2.3 1.1
-
 3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Spinacia oleracea
2.7.2.3 1.28
-
 3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Saccharomyces cerevisiae
2.7.2.3 1.5
-
 3-phospho-D-glycerate at 1.5-13 mM 3-phospho-D-glycerate concentration Spirulina geitleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 46000
-
 x * 46000, SDS-PAGE Saccharomyces cerevisiae
2.7.2.3 46000
-
 x * 46000, SDS-PAGE Spinacia oleracea
2.7.2.3 46000
-
 x * 46000, SDS-PAGE Spirulina geitleri

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Arthrospira platensis
-
-
-
2.7.2.3 Saccharomyces cerevisiae
-
-
-
2.7.2.3 Spinacia oleracea
-
-
-
2.7.2.3 Spirulina geitleri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Arthrospira platensis
2.7.2.3 106fold Spinacia oleracea
2.7.2.3 131fold Saccharomyces cerevisiae
2.7.2.3 36fold Spirulina geitleri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 702
-
 purified enzyme Spinacia oleracea
2.7.2.3 720
-
 purified enzyme Spirulina geitleri
2.7.2.3 785
-
 purified enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years Saccharomyces cerevisiae
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years Spinacia oleracea
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years Spirulina geitleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate
-
 Saccharomyces cerevisiae ATP + 3-phospho-D-glycerate
-
 r
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate
-
 Spinacia oleracea ATP + 3-phospho-D-glycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Arthrospira platensis ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate high specificity for ATP Saccharomyces cerevisiae ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate high specificity for ATP Spinacia oleracea ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate high specificity for ATP Spirulina geitleri ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae ADP + 3-phospho-D-glyceroyl phosphate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Spinacia oleracea ADP + 3-phospho-D-glyceroyl phosphate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Spirulina geitleri ADP + 3-phospho-D-glyceroyl phosphate
-
 r
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Saccharomyces cerevisiae CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Spinacia oleracea CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Spirulina geitleri CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate low activity Spirulina geitleri dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dGTP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae dGDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dGTP + 3-phospho-D-glycerate no activity Spirulina geitleri dGDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dITP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae dIDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dITP + 3-phospho-D-glycerate no activity Spirulina geitleri dIDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate very low activity Spirulina geitleri GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate low activity Spinacia oleracea GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate very low activity Spirulina geitleri IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate low activity Spinacia oleracea IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 additional information no activity with dCTP, dUTP, dTTP Saccharomyces cerevisiae ?
-
 ?
2.7.2.3 additional information no activity with dCTP, dUTP, dTTP Spinacia oleracea ?
-
 ?
2.7.2.3 additional information no activity with dCTP, dUTP, dTTP Spirulina geitleri ?
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate low activity Spinacia oleracea UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate only traces of activity Saccharomyces cerevisiae UDP + 1,3-diphosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 46000, SDS-PAGE Saccharomyces cerevisiae
2.7.2.3 ? x * 46000, SDS-PAGE Spinacia oleracea
2.7.2.3 ? x * 46000, SDS-PAGE Spirulina geitleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Saccharomyces cerevisiae
2.7.2.3 25
-
 assay at Spinacia oleracea
2.7.2.3 25
-
 assay at Spirulina geitleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 6 9.2
-
 Saccharomyces cerevisiae
2.7.2.3 6.5 9.5
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Saccharomyces cerevisiae
2.7.2.3 ADP
-
 Spinacia oleracea
2.7.2.3 ADP
-
 Arthrospira platensis
2.7.2.3 ADP
-
 Spirulina geitleri
2.7.2.3 ATP required as phosphate donor Saccharomyces cerevisiae
2.7.2.3 ATP required as phosphate donor Spinacia oleracea
2.7.2.3 ATP required as phosphate donor Arthrospira platensis
2.7.2.3 ATP required as phosphate donor Spirulina geitleri
2.7.2.3 dATP
-
 Saccharomyces cerevisiae
2.7.2.3 dATP
-
 Spirulina geitleri
2.7.2.3 dGTP
-
 Saccharomyces cerevisiae
2.7.2.3 dITP
-
 Saccharomyces cerevisiae
2.7.2.3 GTP
-
 Saccharomyces cerevisiae
2.7.2.3 GTP
-
 Spinacia oleracea
2.7.2.3 GTP
-
 Spirulina geitleri
2.7.2.3 ITP
-
 Saccharomyces cerevisiae
2.7.2.3 ITP
-
 Spinacia oleracea
2.7.2.3 ITP
-
 Spirulina geitleri
2.7.2.3 UTP
-
 Saccharomyces cerevisiae
2.7.2.3 UTP
-
 Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.3 Spinacia oleracea
-
 4.4 4.3
2.7.2.3 Saccharomyces cerevisiae thin-layer isoelectric focusing 4.4 4.3
2.7.2.3 Spirulina geitleri
-
-
 4.8
2.7.2.3 Saccharomyces cerevisiae thin-layer isoelectric focusing
-
 4.8
2.7.2.3 Saccharomyces cerevisiae thin-layer isoelectric focusing
-
 5.3