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Literature summary extracted from

  • Littlechild, J.A.; Davies, G.J.; Gamblin, S.J.; Watson, H.C.
    Phosphoglycerate kinase from the extreme thermophile Thermus thermophilus. Crystallization and preliminary X-ray data (1987), FEBS Lett., 225, 123-126.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 partial DNA and amino acid sequence determination Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 crystallization from ammonium sulfate precipitation with 37% w/v by hanging or sitting drop vapour diffusion method, protein solution: 4 mg/ml, PIPES buffer, pH 6.8, 10 mM MgCl2, 1 mM ATP, 10% (NH4)2SO4, 22°C, 7-14 days, high resolution X-ray diffraction structure determination and analysis, rotating camera system Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 43000
-
 x * 43000 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate Thermus thermophilus
-
 ATP + 3-phospho-D-glycerate
-
 r
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 ATP + 3-phospho-D-glycerate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Thermus thermophilus
-
-
-
2.7.2.3 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 partial Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate
-
 Thermus thermophilus ATP + 3-phospho-D-glycerate
-
 r
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ATP + 3-phospho-D-glycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Thermus thermophilus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 43000 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 22
-
 assay at Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 85
-
 stable Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.5
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Thermus thermophilus
2.7.2.3 ATP required as phosphate donor Thermus thermophilus