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Literature summary extracted from

  • Schulman, M.D.; Valentino, D.
    Purification and characterization of phosphoglycerate kinase from Fasciola hepatica (1981), Mol. Biochem. Parasitol., 2, 309-321.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid) reversible Fasciola hepatica
2.7.2.3 ADP noncompetitive with respect to ATP and 3-phospho-D-glycerate Fasciola hepatica
2.7.2.3 iodoacetamide irreversible Fasciola hepatica
2.7.2.3 N-ethylmaleimide irreversible Fasciola hepatica
2.7.2.3 p-chloromercuribenzoate reversible Fasciola hepatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.9
-
 ATP
-
 Fasciola hepatica
2.7.2.3 1.26
-
 3-phospho-D-glycerate
-
 Fasciola hepatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 47900
-
 sedimentation equilibrium method Fasciola hepatica

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Fasciola hepatica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 375fold to homogeneity Fasciola hepatica

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.3 ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate sequential mechanism Fasciola hepatica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Fasciola hepatica ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 47900, SDS-PAGE Fasciola hepatica

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Fasciola hepatica
2.7.2.3 ATP required as phosphate donor Fasciola hepatica