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Literature summary extracted from

  • Abdelala, A.T.; Bibb, W.F.; Nainan, O.
    Carbamate kinase from Pseudomonas aeruginosa: purification, characterization, physiological role, and regulation (1982), J. Bacteriol., 151, 1411-1419.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.2 ATP
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 additional information
-
 additional information cooperativity at low ADP concentrations Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.2 additional information activity in HEPES buffer is twice that in potassium citrate buffer at pH 6.0 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 37000
-
 2 * 37000, SDS-PAGE Pseudomonas aeruginosa
2.7.2.2 74000
-
 sucrose density gradient centrifugation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Pseudomonas aeruginosa
-
 PAO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Pseudomonas aeruginosa ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + CO2
-
 Pseudomonas aeruginosa ADP + carbamoyl phosphate
-
 r
2.7.2.2 additional information activity with acetylphosphate is approximately 5% of that with carbamoyl phosphate as phosphoryl donor Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.2 dimer 2 * 37000, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.2 additional information
-
 activity in HEPES buffer is twice that in potassium citrate buffer at pH 6.0 Pseudomonas aeruginosa
2.7.2.2 7
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.2 5 8 pH 5.0: about 50% of maximum activity, pH 8.0: about 60% of maximum activity Pseudomonas aeruginosa