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Literature summary extracted from

  • Raijman, L.; Jones, M.E.
    Carbamate kinase (1973), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 9, 97-119.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.2
-
Enterococcus faecalis

General Stability

EC Number General Stability Organism
2.7.2.2 2-mercaptoethanol protects against heat denaturation Mycoplasma hominis
2.7.2.2 80% loss of activity on dialysis against 0.04 M Tris, pH 8.5, 4°C, 18 h, Streptococcus lactis enzyme Lactococcus lactis
2.7.2.2 ammonium sulfate, 0.5 M, stabilizes against inactivation Enterococcus faecalis
2.7.2.2 unstable in dilute solutions, even at very low temperatures Neurospora crassa
2.7.2.2 unstable in dilute solutions, even at very low temperatures Lactococcus lactis
2.7.2.2 unstable in dilute solutions, even at very low temperatures Serratia marcescens
2.7.2.2 unstable in dilute solutions, even at very low temperatures Enterococcus faecalis
2.7.2.2 unstable in dilute solutions, even at very low temperatures Mycoplasma hominis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.2 Cd2+
-
Enterococcus faecalis
2.7.2.2 Cu2+
-
Enterococcus faecalis
2.7.2.2 iodoacetamide weak Enterococcus faecalis
2.7.2.2 p-hydroxymercuribenzoate
-
Enterococcus faecalis
2.7.2.2 Pb2+
-
Enterococcus faecalis
2.7.2.2 Silver-Tris
-
Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 1.7
-
acetate
-
Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.2 Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Neurospora crassa
2.7.2.2 Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
2.7.2.2 Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
2.7.2.2 Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
2.7.2.2 Bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
2.7.2.2 Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
2.7.2.2 Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
2.7.2.2 Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
2.7.2.2 Co2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
2.7.2.2 Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
2.7.2.2 Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
2.7.2.2 Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
2.7.2.2 Fe2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
2.7.2.2 Mg2+
-
Neurospora crassa
2.7.2.2 Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
2.7.2.2 Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
2.7.2.2 Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
2.7.2.2 Mg2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis
2.7.2.2 Mn2+
-
Neurospora crassa
2.7.2.2 Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Lactococcus lactis
2.7.2.2 Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Serratia marcescens
2.7.2.2 Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Enterococcus faecalis
2.7.2.2 Mn2+ bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+ Mycoplasma hominis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 31000
-
2 * 31000, deduced from amino acid composition Enterococcus faecalis
2.7.2.2 40000 45300 sedimentation equilibrium method Enterococcus faecalis
2.7.2.2 61000
-
sucrose density gradient technique Mycoplasma hominis
2.7.2.2 66000
-
sedimentation data Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.2 additional information Neurospora crassa physiological role ?
-
?
2.7.2.2 additional information Lactococcus lactis physiological role ?
-
?
2.7.2.2 additional information Serratia marcescens physiological role ?
-
?
2.7.2.2 additional information Enterococcus faecalis physiological role ?
-
?
2.7.2.2 additional information Mycoplasma hominis physiological role ?
-
?
2.7.2.2 additional information Enterococcus faecalis D10 physiological role ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Enterococcus faecalis
-
D10
-
2.7.2.2 Enterococcus faecalis D10
-
D10
-
2.7.2.2 Lactococcus lactis
-
-
-
2.7.2.2 Mycoplasma hominis
-
type II strain 07
-
2.7.2.2 Neurospora crassa
-
-
-
2.7.2.2 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2
-
Enterococcus faecalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Neurospora crassa
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Lactococcus lactis
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Serratia marcescens
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Enterococcus faecalis
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Mycoplasma hominis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.2 additional information
-
assay for forward and reverse reaction Neurospora crassa
2.7.2.2 additional information
-
assay for forward and reverse reaction Lactococcus lactis
2.7.2.2 additional information
-
assay for forward and reverse reaction Serratia marcescens
2.7.2.2 additional information
-
assay for forward and reverse reaction Enterococcus faecalis
2.7.2.2 additional information
-
assay for forward and reverse reaction Mycoplasma hominis
2.7.2.2 3100
-
-
Neurospora crassa
2.7.2.2 3100
-
-
Lactococcus lactis
2.7.2.2 3100
-
-
Serratia marcescens
2.7.2.2 3100
-
-
Enterococcus faecalis
2.7.2.2 3100
-
-
Mycoplasma hominis

Storage Stability

EC Number Storage Stability Organism
2.7.2.2 -20°C, 1 week, 50% loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
Neurospora crassa ATP + NH3 + CO2
-
r
2.7.2.2 ADP + carbamoyl phosphate
-
Lactococcus lactis ATP + NH3 + CO2
-
r
2.7.2.2 ADP + carbamoyl phosphate
-
Serratia marcescens ATP + NH3 + CO2
-
r
2.7.2.2 ADP + carbamoyl phosphate
-
Enterococcus faecalis ATP + NH3 + CO2
-
r
2.7.2.2 ADP + carbamoyl phosphate
-
Mycoplasma hominis ATP + NH3 + CO2
-
r
2.7.2.2 ADP + carbamoyl phosphate
-
Enterococcus faecalis D10 ATP + NH3 + CO2
-
r
2.7.2.2 ATP + NH3 + acetate
-
Enterococcus faecalis ADP + ?
-
?
2.7.2.2 ATP + NH3 + acetate
-
Enterococcus faecalis D10 ADP + ?
-
?
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Neurospora crassa ADP + carbamoyl phosphate
-
r
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Lactococcus lactis ADP + carbamoyl phosphate
-
r
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Serratia marcescens ADP + carbamoyl phosphate
-
r
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Enterococcus faecalis ADP + carbamoyl phosphate
-
r
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Mycoplasma hominis ADP + carbamoyl phosphate
-
r
2.7.2.2 ATP + NH3 + CO2 reaction proceeds more readily in direction of ATP synthesis Enterococcus faecalis D10 ADP + carbamoyl phosphate
-
r
2.7.2.2 additional information no activity with GTP, ITP, UTP, CTP Lactococcus lactis ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Neurospora crassa ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Lactococcus lactis ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Serratia marcescens ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Enterococcus faecalis ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Mycoplasma hominis ?
-
?
2.7.2.2 additional information physiological role Neurospora crassa ?
-
?
2.7.2.2 additional information physiological role Lactococcus lactis ?
-
?
2.7.2.2 additional information physiological role Serratia marcescens ?
-
?
2.7.2.2 additional information physiological role Enterococcus faecalis ?
-
?
2.7.2.2 additional information physiological role Mycoplasma hominis ?
-
?
2.7.2.2 additional information some carbamate kinases also utilize acetate Enterococcus faecalis D10 ?
-
?
2.7.2.2 additional information physiological role Enterococcus faecalis D10 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.2.2 dimer 2 * 31000, deduced from amino acid composition Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.2 additional information
-
2-mercaptoethanol protects against heat denaturation Mycoplasma hominis
2.7.2.2 60
-
2 min, 85% loss of activity Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.2 additional information biotin is not involved in reaction system Neurospora crassa
2.7.2.2 additional information biotin is not involved in reaction system Lactococcus lactis
2.7.2.2 additional information biotin is not involved in reaction system Serratia marcescens
2.7.2.2 additional information biotin is not involved in reaction system Enterococcus faecalis
2.7.2.2 additional information biotin is not involved in reaction system Mycoplasma hominis