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Literature summary extracted from

  • Ingram-Smith, C.; Barber, R.D.; Ferry, J.G.
    The role of histidines in the acetate kinase from Methanosarcina thermophila (2000), J. Biol. Chem., 275, 33765-33770.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 wild-type and variant acetate kinases are overproduced in Escherichia coli BL21(DE3) Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 H123A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H152A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H180A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H180R site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H184A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H208A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H60A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H90A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 H94A site-directed mutagenesis Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 diethyldicarbonate
-
 Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.7
-
 ATP pH 7.0, 37°C, mutant H180A, produced in E. coli Methanosarcina thermophila
2.7.2.1 1.4
-
 ATP pH 7.0, 37°C, mutant H180R, produced in E. coli Methanosarcina thermophila
2.7.2.1 1.5
-
 ATP pH 7.0, 37°C, mutant H123A, produced in E. coli Methanosarcina thermophila
2.7.2.1 1.7
-
 ATP pH 7.0, 37°C, mutant H90A, produced in E. coli Methanosarcina thermophila
2.7.2.1 1.9
-
 ATP pH 7.0, 37°C, mutant H208A, produced in E. coli Methanosarcina thermophila
2.7.2.1 2
-
 ATP pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli Methanosarcina thermophila
2.7.2.1 2.7
-
 ATP pH 7.0, 37°C, mutant H184A, produced in E. coli Methanosarcina thermophila
2.7.2.1 2.8
-
 ATP pH 7.0, 37°C, authentic wild-type enzyme Methanosarcina thermophila
2.7.2.1 3
-
 ATP pH 7.0, 37°C, mutant H60A, produced in E. coli Methanosarcina thermophila
2.7.2.1 3.7
-
 ATP pH 7.0, 37°C, mutant H180Q, produced in E. coli Methanosarcina thermophila
2.7.2.1 4.6
-
 ATP pH 7.0, 37°C, mutant H180N, produced in E. coli Methanosarcina thermophila
2.7.2.1 4.7
-
 ATP pH 7.0, 37°C, mutant H180D, produced in E. coli Methanosarcina thermophila
2.7.2.1 5.8
-
 ATP pH 7.0, 37°C, mutant H94A, produced in E. coli Methanosarcina thermophila
2.7.2.1 6.2
-
 ATP pH 7.0, 37°C, mutant H180E, produced in E. coli Methanosarcina thermophila
2.7.2.1 6.3
-
 ATP pH 7.0, 37°C, mutant H180K, produced in E. coli Methanosarcina thermophila
2.7.2.1 10
-
 acetate pH 7.0, 37°C, mutant H180R, produced in E. coli Methanosarcina thermophila
2.7.2.1 13
-
 acetate pH 7.0, 37°C, mutant H180D, produced in E. coli Methanosarcina thermophila
2.7.2.1 14
-
 acetate pH 7.0, 37°C, mutant H180Q, produced in E. coli Methanosarcina thermophila
2.7.2.1 15
-
 acetate pH 7.0, 37°C, mutant H184A, produced in E. coli Methanosarcina thermophila
2.7.2.1 16
-
 acetate pH 7.0, 37°C, mutant H123A, produced in E. coli Methanosarcina thermophila
2.7.2.1 17
-
 acetate pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli Methanosarcina thermophila
2.7.2.1 18
-
 acetate pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli Methanosarcina thermophila
2.7.2.1 19
-
 acetate pH 7.0, 37°C, mutant H180K, produced in E. coli Methanosarcina thermophila
2.7.2.1 20
-
 acetate pH 7.0, 37°C, mutant H60A, produced in E. coli Methanosarcina thermophila
2.7.2.1 22
-
 acetate pH 7.0, 37°C, authentic wild-type enzyme Methanosarcina thermophila
2.7.2.1 33
-
 acetate pH 7.0, 37°C, mutant H208A, produced in E. coli Methanosarcina thermophila
2.7.2.1 56
-
 ATP pH 7.0, 37°C, mutant H180A, produced in E. coli Methanosarcina thermophila
2.7.2.1 66
-
 acetate pH 7.0, 37°C, mutant H94A, produced in E. coli Methanosarcina thermophila
2.7.2.1 330
-
 acetate pH 7.0, 37°C, mutant H90A, produced in E. coli Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Methanosarcina thermophila