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Literature summary extracted from

  • Vigenschow, H.; Schwarm, H.M.; Knobloch, K.
    Purification and properties of an acetate kinase from Rhodopseudomonas palustris (1986), Biol. Chem. Hoppe-Seyler, 367, 951-956.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.0026
-
 acetyl phosphate carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 0.087
-
 ADP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 1.1
-
 ATP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 1.3
-
 UTP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 1.9
-
 CTP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 3.7
-
 ITP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 5.7
-
 GTP carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 40
-
 acetate carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 150
-
 propionate carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 200
-
 Butyrate carried out at various temperatures Rhodopseudomonas palustris
2.7.2.1 420
-
 isobuytyrate carried out at various temperatures Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+ absolute requirement Rhodopseudomonas palustris
2.7.2.1 Mn2+
-
 Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 45000
-
 1 * 45000, SDS-PAGE Rhodopseudomonas palustris
2.7.2.1 46500
-
 gel filtration Rhodopseudomonas palustris
2.7.2.1 47000
-
 gel filtration Rhodopseudomonas palustris
2.7.2.1 48000
-
 gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Rhodopseudomonas palustris
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 3.16
-
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 -24°C, can be stored for several months with only a negligible loss in activity Rhodopseudomonas palustris
2.7.2.1 0-4°C, can be stored for some days with only a negligible loss in activity Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Rhodopseudomonas palustris ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + butyrate
-
 Rhodopseudomonas palustris ADP + butyryl phosphate
-
 r
2.7.2.1 ATP + isobutyrate
-
 Rhodopseudomonas palustris ADP + isobutyryl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Rhodopseudomonas palustris ADP + propionyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Rhodopseudomonas palustris CDP + acetyl phosphate
-
 ?
2.7.2.1 GTP + acetate
-
 Rhodopseudomonas palustris GDP + acetyl phosphate
-
 ?
2.7.2.1 ITP + acetate
-
 Rhodopseudomonas palustris IDP + acetyl phosphate
-
 ?
2.7.2.1 UTP + acetate
-
 Rhodopseudomonas palustris UDP + acetyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 monomer 1 * 45000, SDS-PAGE Rhodopseudomonas palustris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 60
-
 10 min incubation causes a loss in activity of 50% Rhodopseudomonas palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 6.5 7.1
-
 Rhodopseudomonas palustris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.1 Rhodopseudomonas palustris
-
-
 4.9