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Literature summary extracted from

  • Yoshimura, F.
    Purification and characterization of acetate kinase from Veillonella alcalescens ATCC 17748 (1978), Arch. Biochem. Biophys., 189, 424-432.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.1 additional information succinate is unnecessary for the activity of this enzyme Veillonella parvula

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 acetyl phosphate
-
 Veillonella parvula
2.7.2.1 ADP
-
 Veillonella parvula
2.7.2.1 HgCl2
-
 Veillonella parvula
2.7.2.1 iodoacetamide
-
 Veillonella parvula
2.7.2.1 N-ethylmaleimide
-
 Veillonella parvula
2.7.2.1 p-chloromercuribenzoate
-
 Veillonella parvula
2.7.2.1 phosphate
-
 Veillonella parvula
2.7.2.1 potassium hydroxylamine
-
 Veillonella parvula
2.7.2.1 sodium hydroxylamine
-
 Veillonella parvula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.3
-
 ADP pH 7.4, 37°C Veillonella parvula
2.7.2.1 1.2
-
 acetyl phosphate pH 7.4, 37°C Veillonella parvula
2.7.2.1 2
-
 ATP pH 7.4, 37°C Veillonella parvula
2.7.2.1 30
-
 acetate pH 7.4, 37°C Veillonella parvula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+
-
 Veillonella parvula
2.7.2.1 Co2+ can replace Mg2+ Veillonella parvula
2.7.2.1 Mg2+
-
 Veillonella parvula
2.7.2.1 Mn2+
-
 Veillonella parvula
2.7.2.1 Pb2+
-
 Veillonella parvula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 43000
-
 2 * 43000, SDS-PAGE Veillonella parvula
2.7.2.1 66000
-
 gel filtration Veillonella parvula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Veillonella parvula
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Veillonella parvula
-
 ATCC 17748
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Veillonella parvula

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 948
-
-
 Veillonella parvula

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 -18°C, buffer containing 0.25 M sucrose, 40% of initial enzyme activity is lost within 2 months Veillonella parvula
2.7.2.1 -45°C, buffer containing 0.25 M sucrose, seems to be more stable than at -18°C Veillonella parvula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Veillonella parvula ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Veillonella parvula ADP + propionyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Veillonella parvula GDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Veillonella parvula IDP + acetyl phosphate
-
 r
2.7.2.1 additional information enzyme does not react with malate, maleate, bicarbonate, butyrate, tartrate, citrate, formate or succinate Veillonella parvula ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 2 * 43000, SDS-PAGE Veillonella parvula

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 50 70 with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity Veillonella parvula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.2 10
-
 Veillonella parvula

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.1 7.4 8 stable in buffers of pH 7.4 and 8.0, unstable under preincubation at pH 5.5 Veillonella parvula